SmoColi - Achievements

General

• We came up with the idea of a bacterio-quantifier of smoke.
• We worked hard and presented our work during the Regional Jamboree in Amsterdam.
• We received Gold Medal and were invited to advance to the World Championship in Cambridge, USA.
• We documented everything we did in our Wiki Website.

Modeling Achievements

• We made a reaction-diffusion model for the toxic molecule gradient formation in a channel and got a feeling about the feasibility of our ideas and channel dimensions.
• We approximated the gradient analytically and checked that it almost coincides with the numerically obtained one.
• We made a single cell model describing our circuit and verified its operation.
• We performed a robustness analysis of our system to see how the band behaves upon parameter variations and concluded that it is robust and monostable.
• We performed stochastic analysis to see how the GFP band reacts upon noise and concluded one more time about its robustness
• We integrated the single cell model and the reaction-diffusion model in a combined 3D spatiotemporal reaction-diffusion model, simulated for various channel dimensions to get a feeling how the GFP band will move and how the alarm is activated
• We extracted some information about the channel dimensions from the model and used this information in the actual channel design.

Biology

• We improved the pSB6A1 Plasmid to standard 5
• We created the BioBrick LacI<M1> which is a codon modified LacI for multiplasmid operations
• We created two improved version of the P_U BioBrick
• We successfully tested the channel in terms of cell viability and GFP synthesis
• We could established a gradient of a small molecule (IPTG and arabinose) in a tube and also in the microfluidic channel

Microfluidics

• We sistematically came up with the current channel design through a process of developing and refining chain of ideas for different designs.
• We participated in the PDMS channel construction.

Human Practices

• We presented Synthetic Biology and iGEM to our fellow students in the CBB get together afternoon.
• We presented iGEM and our project to the general public on the BSSE Open House Day through a poster and a slide show
• We also made a survey with critical questions related to Synthetic Biology and iGEM, asked people to fill it out and analyzed the results.
• ETH GLOBE ?

Miscelaneous

• Laura managed to move the Dropbox folder to another location, thus making it look empty for the others.
• Sabine accidentally modified an other teams wiki
• We had fun!

Section A

Test

Subsection

More testing