Team:ETH Zurich/Achievements
From 2011.igem.org
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* We improved the pSB6A1 Plasmid to standard 5: [http://partsregistry.org/Part:BBa_K625005 pSB6A5] | * We improved the pSB6A1 Plasmid to standard 5: [http://partsregistry.org/Part:BBa_K625005 pSB6A5] | ||
- | * We created the BioBrick LacI<sub>M1</sub> which is a codon modified LacI for multiplasmid operations | + | * We created the [http://partsregistry.org/Part:BBa_K625000 BioBrick LacI<sub>M1</sub>] which is a codon modified LacI for multiplasmid operations |
* We created two improved version of the P<sub>U</sub> BioBrick | * We created two improved version of the P<sub>U</sub> BioBrick | ||
* We successfully tested the channel in terms of cell viability and GFP synthesis | * We successfully tested the channel in terms of cell viability and GFP synthesis |
Revision as of 14:03, 27 October 2011
SmoColi - Achievements
General
- We came up with the idea of a bacterio-quantifier of smoke.
- We worked hard and presented our work during the Regional Jamboree in Amsterdam.
- We received Gold Medal and were invited to advance to the World Championship in Cambridge, USA.
- We documented everything we did in our Wiki Website.
Modeling Achievements
- We made a reaction-diffusion model for the toxic molecule gradient formation in a channel and got a feeling about the feasibility of our ideas and channel dimensions.
- We approximated the gradient analytically and checked that it almost coincides with the numerically obtained one.
- We made a single cell model describing our circuit and verified its operation.
- We performed a robustness analysis of our system to see how the band behaves upon parameter variations and concluded that it is robust and monostable.
- We performed stochastic analysis to see how the GFP band reacts upon noise and confirmed one more time its robustness
- We integrated the single cell model and the reaction-diffusion model in a combined 3D spatiotemporal reaction-diffusion model, simulated for various channel dimensions to get a feeling how the GFP band will move and how the alarm is activated
- We extracted some information about the channel dimensions from the model and used this information in the actual channel design.
Biology
- We improved the pSB6A1 Plasmid to standard 5: pSB6A5
- We created the BioBrick LacIM1 which is a codon modified LacI for multiplasmid operations
- We created two improved version of the PU BioBrick
- We successfully tested the channel in terms of cell viability and GFP synthesis
- We could established a gradient of a small molecule (IPTG and arabinose) in a tube and also in the microfluidic channel
Microfluidics
- We sistematically came up with the current channel design through a process of developing and refining chain of ideas for different designs.
- Modeling help to improved the channel design
- We participated in the PDMS channel construction.
Human Practices
- We presented Synthetic Biology and iGEM to our fellow students in the CBB get together afternoon.
- We presented iGEM and our project to the general public on the BSSE Open House Day through a poster and a slide show.
- We also made a survey with critical questions related to Synthetic Biology and iGEM, asked people to fill it out and analyzed the results.
- ETH GLOBE and the NZZ (Neue Zeitung Zürich) will write about our participation at ????
Personal achievements
- xx managed to move the Dropbox folder to another location, thus making it look empty for the others.
- xx accidentally modified an other teams wiki for 1 min
- xx slept the whole boat trip in Amsterdam
- xx found a mistake in the PowerPoint in the morning before the presentation ("And the channel turns read")
- We had fun!