Team:EPF-Lausanne/Tools/Microfluidics/HowTo2

From 2011.igem.org

(Difference between revisions)
(Details of connections)
 
(26 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:EPF-Lausanne/Templates/MicrofluidicsHeader|title=Microfluidics how-to part II: build your setup}}
+
{{:Team:EPF-Lausanne/Templates/MicrofluidicsHeader|title=Microfluidics How-To Part II: Building Your Setup}}
-
 
+
-
    To be done: describe how to design and make a chip. Or how to order one.
+
[[File:EPFL-Basic-setup.jpg|thumb|right|300px|A basic computer-controlled microfluidics setup. Note the compressed air input split into two sides, both fed through a pressure regulator. The left side is the low-pressure manifold for the flow layer. The right side is the high-pressure solenoid array for the control layer.]]
[[File:EPFL-Basic-setup.jpg|thumb|right|300px|A basic computer-controlled microfluidics setup. Note the compressed air input split into two sides, both fed through a pressure regulator. The left side is the low-pressure manifold for the flow layer. The right side is the high-pressure solenoid array for the control layer.]]
-
Microfludic chips are nothing but a piece of moulded rubber. To actually get use them, an external setup of tubing, compressed air, and valves is needed to flow in fluids and actuate the on-chip valves. To see what's happening, you'll also need some form of microscope. No matter the application of the chip, whether it is designed to study fluid mechanics, to characterise protein-DNA interaction, or even cultivate bacteria and nematodes, the external setup remains essentially the same.
+
Microfludic chips are nothing but a piece of moulded rubber. To actually get something out of them, an external setup of tubing, compressed air, and valves is needed to flow in fluids and actuate the on-chip valves. To see what's happening, you'll also need some form of microscope. No matter the application of the chip, whether it is designed to study fluid mechanics, to characterise protein-DNA interaction, or even cultivate bacteria and nematodes, the external setup remains essentially the same.
-
 
+
-
== Making a chip ==
+
-
 
+
-
    TODO: outline wafer fabrication and chip fab. Or just refer to Stanford chip ordering service.
+
== A basic microfluidics control setup ==
== A basic microfluidics control setup ==
-
=== Injecting fluids: mains pressure and tubing, pressure regulators ===
+
Building the setup is relatively straightforward, once you have determined which components are needed and their purpose, and once you have collected the parts. Connecting the components is basic plumbing: just remember to wrap plumber's tape around all metallic screw threads, and then connect the pressure regulators in the correct orientation. With this in mind, we'll go through the parts of a microfluidics setup one by one, and from there you should be able to build your own.
 +
 
 +
=== Injecting fluids: compressed air and tubing, pressure regulators ===
[[File:EPFL-Flow-manifold.jpg|thumb|right|Close-up of the flow-layer tube: connected to a manifold, and plugged into the chip using a tubular pin]]
[[File:EPFL-Flow-manifold.jpg|thumb|right|Close-up of the flow-layer tube: connected to a manifold, and plugged into the chip using a tubular pin]]
Line 20: Line 16:
On the other end, the tubes are plugged into a manifold, in turn supplied with air at about 0.2 bar (3 psi), as set by a pressure regulator. The fluid is thus forced into the channels by the compressed air. A syringe can also be used to fill the chip, but it is hard to keep an even pressure (plus you quickly run out of hands).
On the other end, the tubes are plugged into a manifold, in turn supplied with air at about 0.2 bar (3 psi), as set by a pressure regulator. The fluid is thus forced into the channels by the compressed air. A syringe can also be used to fill the chip, but it is hard to keep an even pressure (plus you quickly run out of hands).
-
=== Controlling flow: microfluidic valves and 3-way valves. ===
+
=== Controlling flow: on-chip valves and 3-way valves. ===
Our chips have a second ''control layer'' above or below the main ''flow layer''. The layers are separated by a thin membrane of PDMS, and their channels overlap in specific locations. When channels of the control layer are pressurized, the membrane bends into the flow layer and blocks it. This creates a microfluidic 'on-chip' valve.
Our chips have a second ''control layer'' above or below the main ''flow layer''. The layers are separated by a thin membrane of PDMS, and their channels overlap in specific locations. When channels of the control layer are pressurized, the membrane bends into the flow layer and blocks it. This creates a microfluidic 'on-chip' valve.
-
[[File:EPFL-Solenoids.jpg|thumb|left|Our array of solenoid valves, on a manifold. These allow individual pressurising of on-chip valves of the control layer.]]
+
[[File:EPFL-Solenoids.jpg|thumb|left|Our array of solenoid valves, on a manifold. These allow individual pressurisation of on-chip valves of the control layer.]]
The channels of the control layer are filled in the same way as the flow layer, but require much higher pressure to bend the membrane. We typically used about 2 bar or 30 psi. Each independant valve in the control layer is pressurised by a different tube, regulated by a three-way valve. These switch between high pressure (from mains pressure, and the pressure regulator) to low pressure (atmospheric pressure), and hence allow quick toggling of the microfluidic valve between the open (unpressurised) and closed (pressurised) state. Three way valves come in manually- or electrically-controlled flavours. The manual kind is simpler and more reliable; we use them for the MITOMI chips. The electric kind (more specifically ''solenoid'' valves) we use for the web-controlled setup.
The channels of the control layer are filled in the same way as the flow layer, but require much higher pressure to bend the membrane. We typically used about 2 bar or 30 psi. Each independant valve in the control layer is pressurised by a different tube, regulated by a three-way valve. These switch between high pressure (from mains pressure, and the pressure regulator) to low pressure (atmospheric pressure), and hence allow quick toggling of the microfluidic valve between the open (unpressurised) and closed (pressurised) state. Three way valves come in manually- or electrically-controlled flavours. The manual kind is simpler and more reliable; we use them for the MITOMI chips. The electric kind (more specifically ''solenoid'' valves) we use for the web-controlled setup.
Line 41: Line 37:
The presence of bubbles in a channel increases its fluidic resistance, and therefore perturbates or even blocks flow. To avoid this, the first step in running a chip is ''priming'': filling all the channels with fluid, then closing all the micro-valves and keeping pressure applied, until any air remaining in the chip diffuses through the chip walls and disappears.
The presence of bubbles in a channel increases its fluidic resistance, and therefore perturbates or even blocks flow. To avoid this, the first step in running a chip is ''priming'': filling all the channels with fluid, then closing all the micro-valves and keeping pressure applied, until any air remaining in the chip diffuses through the chip walls and disappears.
-
Once all the channels are filled, the experiments can be ran. For the worm chip, we would prime it with buffer, then introduce the worm when the chip is filled. For the MITOMI chip, we would flow in proteins and whatever.
+
Once all the channels are filled, the experiments can begin. For the worm chip, we would prime the device with buffer, then introduce the worm after the chip is filled. For the MITOMI chip, we would flow in various biomolecules (neutravidin, bovine serum albumin, antibodies, cell-free extracts) and DNA.
 +
 
 +
== Details of connections ==
 +
 
 +
Below are four images detailing how the tubing and electronics in our setup are connected, and the role of each component.
 +
 
 +
A printed circuit board (PCB) is used to connect the solenoid valves to the relays on the easyDAQ, and power them. The solenoids are connected to the PCB via two flat cables, one soldered to the red wires, one soldered to the black wires (they are 14-wire cables, with two wires removed). The other end of each cable is fitted with a 14-pin connector. The PCB is powered by a 24 V power supply, and distributes the power to each solenoid through the relays on the EasyDAQ. Therefore, when a relay is open, a 24 V load is applied to the corresponding solenoid (wich opens it). When the relay is closed, both leads of the solenoid are grounded (which closes it).
 +
 
 +
[[File:EPFL-Setup.png|thumb|left|140px|Annotated picture of the fluidics setup. The role of each component is explained. Not shown: since the picture was taken, manometers were added to the pressure regulators.]]
 +
[[File:EPFL-PCB.png|thumb|left|140px|Close-up of the PCB that connects the valves to the EasyDAQ.]]
 +
[[File:EPFL-relay_schematic.jpg|thumb|left|140px|How the solenoids are switched between ground and 24 V by the relays. Only shown for four relays; our setup has twelve connected identically.]]
 +
[[File:EPFL-EasyDAQ.jpg|thumb|left|140px|The EasyDAQ: 24 USB-controlled relays. The relays are connected to the PCB just by wires fixed in the screw-in headers.]]
 +
 
 +
== Complete parts list ==
 +
 
 +
All the parts used for the pneumatics, with approximate cost in Swiss francs, are listed below. The microscope is an additional cost, but most labs would have one already. The Mac Pro is a lone from EPFL's Biomedical Imaging Group, and the Thinkpad is an oldie that was lying around.
 +
 
 +
Savings could be made on the pressure regulators, on the valves, and on the EasyDAQ - most setups are better off with manual valves anyway.
 +
{|
 +
! Part || Supplier || Qty || Unit cost [CHF] || Subtotal [CHF]
 +
|-
 +
| Pressure Regulator || Bellofram || 2 || 186.00 || 372.00
 +
|-
 +
| Manometer 0 - 4.5 bar || Mosch || 2 || 11.20 || 22.40
 +
|-
 +
| EasyDAQ USB24mx || EasyDAQ || 1 || 230.00 || 230.00
 +
|-
 +
| 12-Solenoid manifold (part S08-1557) || Pneumadyne || 1 || 245.00 || 245.00
 +
|-
 +
| Luer barbed adapter, 1/16" (part EW-45503-00) || Cole-Parmer || 1 || 9.20 || 9.20
 +
|-
 +
| Luer valve manifold (part EW-06464-87) || Cole-Parmer || 1 || 10.30 || 10.30
 +
|-
 +
| Luer lock needles .6 mm ID, 12.7 mm length || Dosieren.net || 100 || 0.28 || 28.00
 +
|-
 +
| Tygon .02" tubing, 100 ft || Cole-Parmer || 1 || 50 || 50.00
 +
|-
 +
| PE Tubing (6 mm x 4 mm ODxID), 100 m || Serto || 1 || 30 || 30.00
 +
|-
 +
| Push-to-connect fittings, for 6 mm OD (various) || Riegler || ~10 || [varies] || ~15.00
 +
|-
 +
| Teflon tape, 6 mm || Serto || 1 || 10.92 || 10.92
 +
|-
 +
! Total ||  ||  ||  || 1007.82
 +
|}
{{:Team:EPF-Lausanne/Templates/Footer}}
{{:Team:EPF-Lausanne/Templates/Footer}}

Latest revision as of 12:36, 23 October 2011