Team:EPF-Lausanne/Tools/MITOMI

From 2011.igem.org

(Difference between revisions)
(MITOMI scans explanation)
(MITOMI scans explanation)
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For data collection the fluorescence intensity measured under the button is normalised to the background and the DNA/protein ratio is calculated. This ratio is ploted against the intensity of the fluorescence of DNA in solution to make the saturation curves. And the Kd for each sequence is calculated from these curves by fitting the next equation:
For data collection the fluorescence intensity measured under the button is normalised to the background and the DNA/protein ratio is calculated. This ratio is ploted against the intensity of the fluorescence of DNA in solution to make the saturation curves. And the Kd for each sequence is calculated from these curves by fitting the next equation:
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[[File:EPFL2011_MITOMI_Kd_fiting_formula.png|150px]]
+
[[File:EPFL2011_MITOMI_Kd_fiting_formula.png|180px]]
[[File:EPFL2011_MITOMI_Example_Saturation_curve_fited.png|400px]]
[[File:EPFL2011_MITOMI_Example_Saturation_curve_fited.png|400px]]

Revision as of 23:43, 21 September 2011