Team:EPF-Lausanne/Todo

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== General ==
== General ==
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* Make competent cells
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* <s>Prepare antibiotic aliquotes</s>
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* Edit the Google doc inventory, SPECIFY THE NAMES you put on the tubes if they are not straightforward!
* Edit the Google doc inventory, SPECIFY THE NAMES you put on the tubes if they are not straightforward!
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* Keep an eye on the SOC and LB medium stocks to be sure they are not contaminated
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* Keep an eye on the stocks of agar plates
== Supplies ==
== Supplies ==
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=== Plasmids ===
=== Plasmids ===
* <s> Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP? </s>
* <s> Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP? </s>
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* <s>Design Gibson primers to assemble the three different plasmids.</s>
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* <s>Design Gibson primers to assemble the different plasmids.</s>
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* <s>Receive said primers</s>
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* Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid" (?).
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* Think of new assemblies we want to make (pTet with RFP, for example)
* Think of new assemblies we want to make (pTet with RFP, for example)
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Reporter plasmids:
* J61002 plasmid:  
* J61002 plasmid:  
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** <s>adding pTet: OK, colony PCR works</s>
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** <s>add pTet: OK, colony PCR works</s>
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** <s>adding tetR with const promoter: troubles with amplifying the parts => left aside for the moment </s>
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** add <s>Plac-RFP</s> or Plac-lysis
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** adding LacI under Ptet + RFP under Plac: only one colony PCR, but failed -> need to de Gibson again
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** add Ptet-LacI subsequently: not needed anymore
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** adding LacI under Ptet + lysis cassette under Plac: colony PCR failed -> need to de Gibson again
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* <s>J23019 plasmid: PCR failed so far => test new plasmids for the LacI plasmid </s>
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TetR plasmid
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*<s>pSB3C5 plasmid: glycerol stock, pconst-TetR added, transformation failed => try with pSB3K1</s>
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* J23019 plasmid: <s>PCR failed so far => test new plasmids for the TetR plasmid </s>
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* pSB3K1 plasmid: colony PCR worked
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* pSB3C5 plasmid: <s> Gibson transformation failed => try with pSB3K1</s>
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* pSB3K1 plasmid:
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** <s>add Pconst-TetR </s>
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** <s> cut out RFP and religate </s>
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** <s>sequence</s>
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** <s>add Ptet-LacI</s>
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** <s>sequence</s>
Specifically:
Specifically:
* Run PCRs and gels on existing sequences (plasmid backbone, TetR gene, RFP) to prepare the parts
* Run PCRs and gels on existing sequences (plasmid backbone, TetR gene, RFP) to prepare the parts
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* extract the correct parts from the gel and purify (or use directly PCR products, after purification)
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* extract the correct parts from the gel and purify (or purify directly PCR products)
* Make a Gibson reaction
* Make a Gibson reaction
* Transform cells -> plates -> liquid cultures
* Transform cells -> plates -> liquid cultures
* From the plates, make a colony PCR
* From the plates, make a colony PCR
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* Miniprep the plasmids from cultures, check  if Gibson is ok by doing a digestion
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* Miniprep the plasmids from cultures, send for sequencing
=== TetR mutants ===
=== TetR mutants ===
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== Wiki ==
== Wiki ==
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=== requirements ===
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# <s>The team's project must be documented on the iGEM Wiki by the deadline.</s>
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# <s>All team pages on the iGEM Wiki must be in the team namespace.</s>
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# The team's project description must be documented on their iGEM Team Wiki by the deadline. The project description should be 1-3 paragraphs and only needs to include an up-to-date explanation of the project.
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# <s>The team's wiki must include a Safety page and an attributions section.</s> Safety in "Considerations" and Attributions in "Our project"
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# <s>All iGEM Team Wikis must include on the front page the iGEM logo and a visible link back to the iGEM 2011 Wiki Main Page</s>.
=== General ===
=== General ===
Make sure we comply with the [[Requirements]]!
Make sure we comply with the [[Requirements]]!
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* Make a banner.
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* <s>Make a banner</s>.
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* Write-up team presentation
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* <s>Write-up team presentation</s>
* Upload our initial research about transcription factors
* Upload our initial research about transcription factors
** Document our choice. We spend a few weeks on this, show how we decided. One criteria for tetR was the amount of research already done for it. Another was low cost...
** Document our choice. We spend a few weeks on this, show how we decided. One criteria for tetR was the amount of research already done for it. Another was low cost...
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* Create '''data''' page
* Create '''data''' page
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It might make sense to merge the "resources" and "tools" menus into "Background", where we discuss our choices of transcription factor, and present our tools: Microfluidics, Gibson assembly, etc.  
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It might make sense to merge the "resources" and "tools" menus into "Background", where we discuss our choices of transcription factor, and present our tools: Microfluidics, Gibson assembly, etc.
=== Assembly ===
=== Assembly ===
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** '''Include an easy printing option''' seriously work on printing template.
** '''Include an easy printing option''' seriously work on printing template.
* Write a new protocol!
* Write a new protocol!
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* Upload "chemostat" protocols
 
=== Front Page ===
=== Front Page ===
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<ol id="criterialist">
<ol id="criterialist">
<li><s>Team registration</s></li>
<li><s>Team registration</s></li>
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<li>Complete Project Summary form</li>
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<li><s>Complete Project Summary form</s></li>
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<s><li>Team Wiki</li></s>
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<li><s>Team Wiki</s></li>
<li>Present a poster and a talk at the iGEM Jamboree</li>
<li>Present a poster and a talk at the iGEM Jamboree</li>
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<li>At least one new submitted and well-characterized standard BioBrick Part or Device. A new application of and outstanding documentation (quantitative data showing the Part’s/ Device’s function) of a previously existing BioBrick part in the “Experience” section of that BioBrick’s Registry entry also counts.</li></ol>
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<li><s>At least one new submitted and well-characterized standard BioBrick Part or Device. A new application of and outstanding documentation (quantitative data showing the Part’s/ Device’s function) of a previously existing BioBrick part in the “Experience” section of that BioBrick’s Registry entry also counts.</s></li></ol>
<b>Silver</b>: In addition to the Bronze Medal requirements...<br>
<b>Silver</b>: In addition to the Bronze Medal requirements...<br>
<ol id="criterialist">
<ol id="criterialist">
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<li>Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected</li>
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<li><s>Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected</s></li>
<li>Characterize the operation of at least one new BioBrick Part or Device and enter this information in the “Main Page” section of that Part’s/Device’s Registry entry.</li></ol>
<li>Characterize the operation of at least one new BioBrick Part or Device and enter this information in the “Main Page” section of that Part’s/Device’s Registry entry.</li></ol>

Latest revision as of 01:06, 21 September 2011

Retrieved from "http://2011.igem.org/Team:EPF-Lausanne/Todo"