Team:EPF-Lausanne/Protocols/TetR

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(PCR Cycle (30 cycles))
 
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{{:Team:EPF-Lausanne/Templates/Header|title=Linear template- TetR}}
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{{:Team:EPF-Lausanne/Templates/ProtocolHeader|title=Linear template- TetR}}
Two step extension PCR
Two step extension PCR
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== PCR Cycle (10 cycles) ==
== PCR Cycle (10 cycles) ==
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<b>1.</b> 98°C for 30s
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:<b>1.</b> 98°C for 30s
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<b>2.</b> 98°C for 7.5 seconds
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:<b>2.</b> 98°C for 7.5 seconds
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<b>3.</b> 58°C for 20 sec  
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:<b>3.</b> 58°C for 20 sec  
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<b>4.</b> 72°C for 15 sec;  
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:<b>4.</b> 72°C for 15 sec;  
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<b>5.</b> 72°C for 5 min
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:<b>5.</b> 72°C for 5 min
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<b>6.</b> 4°C "forever"
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:<b>6.</b> 4°C "forever"
== Materials ==
== Materials ==
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== PCR Cycle (30 cycles) ==
== PCR Cycle (30 cycles) ==
-
<b>1.</b> 98°C for 30s
+
:<b>1.</b> 98°C for 30s
-
<b>2.</b> 98°C for 7.5 seconds
+
:<b>2.</b> 98°C for 7.5 seconds
-
<b>3.</b> 53°C for 20 sec
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:<b>3.</b> 53°C for 20 sec
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<b>4.</b> 72°C for 15 sec;  
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:<b>4.</b> 72°C for 15 sec;  
-
<b>5.</b> 72°C for 5 min
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:<b>5.</b> 72°C for 5 min
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<b>6.</b> 4°C "forever"
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:<b>6.</b> 4°C "forever"
{{:Team:EPF-Lausanne/Templates/Footer}}
{{:Team:EPF-Lausanne/Templates/Footer}}

Latest revision as of 08:48, 15 July 2011