• Introduction
  • Home
  • The Team
  • Photo Gallery
• Our Project
  • Results Summary
  • Selection System
  • In-Vitro Characterization
  • In-Vivo Characterization
  • Microfluidics
  • Data
  • Attributions
• Tools
  • Gibson Assembly
  • MITOMI
• Notebook
  • Protocols
  • May
  • June
  • July
  • August
  • September
  • October
• Considerations
  • Human Practices
  • Safety
• Acknowledgements
  • Sponsors
  • Partners

PDMS two layer device fabrication

made on 16.05.2011

Materials

• flow and control layer molds
• Sylgard 184 silicone elastomer kit: Base part and a Curing agent
• TMCS

Method

• Place molds into a TMCS vapor chamber

• Control layer mixture: 20g Base + 4g Curing agent

• Mix for 1 minute, degas for 2 minutes (standard protocol)

• pour onto control layer mold and place mold in vacuum chamber

• Flow layer mixture: 20g Base + 1g Curing agent

• Mix for 1 minute and degas for 2 minutes (standard protocol)

• Spin coat onto flow layer at 2200-2400rpm for 35secs (so far you can use my protocol on a spin coater)

• Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some )

• Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!)

• Remove casts from oven, cut out control layer, punch holes, and align to flow layer

• Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing time)

• Remove devices from oven and punch flow layer holes