Team:EPF-Lausanne/Protocols/PDMS two layer device fabrication
From 2011.igem.org
PDMS two layer device fabrication
Back to protocols.made on 16.05.2011
Materials
- flow and control layer molds
- Sylgard 184 silicone elastomer kit: Base part and a Curing agent
- TMCS
Method
- Place moulds into a TMCS vapor chamber for twenty minutes
- Control layer mixture: 20g Base + 4g Curing agent
- Mix for 1 minute, degas for 2 minutes (standard protocol)
- Pour onto control layer mold and place mold in vacuum chamber for at least 20min
- Flow layer mixture: 20g Base + 1g Curing agent
- Mix for 1 minute and degas for 2 minutes (standard protocol)
- Spin coat onto flow layer at 2200-2400rpm for 35secs
- Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some )
- Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!)
- Remove casts from oven, cut out control layer, punch holes, and align to flow layer
- Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing time)
- Remove devices from oven, cut them out and punch flow layer holes