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Team:EPF-Lausanne/Protocols
From 2011.igem.org
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* [[Team:EPF-Lausanne/Protocols/Gibson assembly|Gibson assembly]]. | * [[Team:EPF-Lausanne/Protocols/Gibson assembly|Gibson assembly]]. | ||
* [[Team:EPF-Lausanne/Protocols/TetR|Linear template- TetR]]. | * [[Team:EPF-Lausanne/Protocols/TetR|Linear template- TetR]]. | ||
| - | * [[Team:EPF-Lausanne/Protocols/TetR | + | * [[Team:EPF-Lausanne/Protocols/TetR Extension PCR|tetR Overlap Extension PCR]]: induce specific mutations on tetR linear template. |
* [[Team:EPF-Lausanne/Protocols/|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification). | * [[Team:EPF-Lausanne/Protocols/|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification). | ||
Revision as of 07:22, 14 July 2011
Protocols
Contents |
Molecular Biology
Products and stock preparation
- Primer preparation: initial dilution of ordered primers.
- Competent Cells: prepare cells for plasmid uptake.
- Competent Cells. Protocol II (Inoue method).
Cloning, assembly, and mutations
- Transformation: introduce foreign plasmid into competent cells.
- Gibson assembly.
- Linear template- TetR.
- tetR Overlap Extension PCR: induce specific mutations on tetR linear template.
- Agarose gel electrophoresis: DNA samples analysis (can be followed by band purification).
DNA recovery
- Plasmid preparation - Miniprep: recover plasmids from a bacterial culture.
- Gel purification: recover DNA separated by electrophoresis
Microfluidics
- PDMS two layer device fabrication
- MITOMI: Protein – DNA interactions
- Chemostat cell culture
- Klenow dsDNA synthesis
