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Team:EPF-Lausanne/Protocols
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* [[Team:EPF-Lausanne/Protocols/Competent cells|Competent Cells]]: prepare cells for plasmid uptake. | * [[Team:EPF-Lausanne/Protocols/Competent cells|Competent Cells]]: prepare cells for plasmid uptake. | ||
* [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]]. | * [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]]. | ||
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| - | === | + | === Cloning, assembly, and mutations === |
| + | * [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]: introduce foreign plasmid into competent cells. | ||
* [[Team:EPF-Lausanne/Protocols/Gibson assembly|Gibson assembly]]. | * [[Team:EPF-Lausanne/Protocols/Gibson assembly|Gibson assembly]]. | ||
* [[Team:EPF-Lausanne/Protocols/TetR|Linear template- TetR]]. | * [[Team:EPF-Lausanne/Protocols/TetR|Linear template- TetR]]. | ||
| - | + | * [[Team:EPF-Lausanne/Protocols/TetR Mutation PCR|tetR mutation PCR]]: induce specific mutations on tetR linear template. | |
| - | + | * [[Team:EPF-Lausanne/Protocols/|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification). | |
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| - | * [[Team:EPF-Lausanne/Protocols/TetR Mutation PCR|tetR mutation PCR]]: induce | + | |
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=== DNA recovery === | === DNA recovery === | ||
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* [[Team:EPF-Lausanne/Protocols/Miniprep|Plasmid preparation - Miniprep]]: recover plasmids from a bacterial culture. | * [[Team:EPF-Lausanne/Protocols/Miniprep|Plasmid preparation - Miniprep]]: recover plasmids from a bacterial culture. | ||
* [[Team:EPF-Lausanne/Protocols/Gel purification|Gel purification]]: recover DNA separated by electrophoresis | * [[Team:EPF-Lausanne/Protocols/Gel purification|Gel purification]]: recover DNA separated by electrophoresis | ||
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== Microfluidics == | == Microfluidics == | ||
Revision as of 14:24, 13 July 2011
Protocols
Contents |
Molecular Biology
Products and stock preparation
- Primer preparation: initial dilution of ordered primers.
- Competent Cells: prepare cells for plasmid uptake.
- Competent Cells. Protocol II (Inoue method).
Cloning, assembly, and mutations
- Transformation: introduce foreign plasmid into competent cells.
- Gibson assembly.
- Linear template- TetR.
- tetR mutation PCR: induce specific mutations on tetR linear template.
- Agarose gel electrophoresis: DNA samples analysis (can be followed by band purification).
DNA recovery
- Plasmid preparation - Miniprep: recover plasmids from a bacterial culture.
- Gel purification: recover DNA separated by electrophoresis
Microfluidics
- PDMS two layer device fabrication
- MITOMI: Protein – DNA interactions
- Chemostat cell culture
- Klenow dsDNA synthesis
