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Team:EPF-Lausanne/Protocols
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* [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]. | * [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]. | ||
* [[Team:EPF-Lausanne/Protocols/Primers preparation|Primers preparation]]. | * [[Team:EPF-Lausanne/Protocols/Primers preparation|Primers preparation]]. | ||
| + | * [[Team:EPF-Lausanne/Protocols/Gibson assembly|Gibson assembly]]. | ||
| + | * [[Team:EPF-Lausanne/Protocols/Gel purification|Gel purification]]. | ||
== Microfluidics == | == Microfluidics == | ||
Revision as of 09:02, 12 July 2011
Protocols
Molecular Biology
- Plasmid preparation - Miniprep: recover plasmids from a bacterial culture.
- Linear template- TetR.
- Agarose gel electrophoresis: DNA samples analysis (can be followed by band purification).
- Competent Cells.
- Competent Cells. Protocol II (Inoue method).
- Transformation.
- Primers preparation.
- Gibson assembly.
- Gel purification.
Microfluidics
- PDMS two layer device fabrication
- MITOMI: Protein – DNA interactions
- Chemostat cell culture
- Klenow dsDNA synthesis
