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Team:EPF-Lausanne/Protocols
From 2011.igem.org
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* [[Team:EPF-Lausanne/Protocols/|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification). | * [[Team:EPF-Lausanne/Protocols/|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification). | ||
* [[Team:EPF-Lausanne/Protocols/Competent cells|Competent Cells]]. | * [[Team:EPF-Lausanne/Protocols/Competent cells|Competent Cells]]. | ||
| - | * [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II]]. | + | * [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]]. |
* [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]. | * [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]. | ||
Revision as of 09:48, 8 July 2011
Protocols
Molecular Biology
- Plasmid preparation - Miniprep: recover plasmids from a bacterial culture.
- Linear template- TetR.
- Agarose gel electrophoresis: DNA samples analysis (can be followed by band purification).
- Competent Cells.
- Competent Cells. Protocol II (Inoue method).
- Transformation.
Microfluidics
- PDMS two layer device fabrication
- MITOMI: Protein – DNA interactions
- Chemostat cell culture
- Klenow dsDNA synthesis
