Team:EPF-Lausanne/Protocols

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(Molecular Biology)
(Cloning, assembly, and mutations)
 
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== Molecular Biology ==
== Molecular Biology ==
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* [[Team:EPF-Lausanne/Protocols/Miniprep|Plasmid preparation - Miniprep]]: recover plasmids from a bacterial culture.
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=== Products and stock preparation ===
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* [[Team:EPF-Lausanne/Protocols/Primers preparation|Primer preparation]]: initial dilution of ordered primers.
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* [[Team:EPF-Lausanne/Protocols/Competent cells|Competent Cells]]: prepare cells for plasmid uptake.
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* [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]].
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* [[Team:EPF-Lausanne/Protocols/Glycerol stock|Glycerol stock]]: stock transformed cells at -80°C.
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* [[Team:EPF-Lausanne/Protocols/Agar Plates|Agar plate preparation]]: preparing agar plates for cell cultures.
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* [[Team:EPF-Lausanne/Protocols/Autoclave|Autoclave]]: to sterilize solutions and glassware.
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=== Cloning, assembly, and mutations ===
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* [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]: introduce foreign plasmid into competent cells.
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* [[Team:EPF-Lausanne/Protocols/Plating|Plating]]: plate transformed cells
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* [[Team:EPF-Lausanne/Protocols/Liquid cultures|Liquid cultures]]: when cells have grown on plates, put them in liquid cultures
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* [[Team:EPF-Lausanne/Protocols/Gibson assembly|Gibson assembly]].
* [[Team:EPF-Lausanne/Protocols/TetR|Linear template- TetR]].
* [[Team:EPF-Lausanne/Protocols/TetR|Linear template- TetR]].
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* [[Team:EPF-Lausanne/Protocols/|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification).
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* [[Team:EPF-Lausanne/Protocols/TetR Extension PCR|tetR Overlap Extension PCR]]: induce specific mutations on tetR linear template.
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* [[Team:EPF-Lausanne/Protocols/Site-specific mutagenesis|tetR Site-specific mutagenesis]]: induce site-specific mutations on a plasmid containing tetR.
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* [[Team:EPF-Lausanne/Protocols/Gel_Electrophoresis|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification).
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* [[Team:EPF-Lausanne/Protocols/Colony PCR|Colony PCR]]: Analyze the plated transformed cells, to see if Gibson assembly worked
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* [[Team:EPF-Lausanne/Protocols/T7-ext|T7 extension PCR]]: Put T7 promoter upstream of a gene
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* [[Team:EPF-Lausanne/Protocols/bluntends|Blunt-End Cloning]]: Making Blunt Ends
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=== DNA recovery ===
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* [[Team:EPF-Lausanne/Protocols/Miniprep|Plasmid preparation - Miniprep]]: recover plasmids from a bacterial culture.
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* [[Team:EPF-Lausanne/Protocols/Gel purification|Gel purification]]: recover DNA separated by electrophoresis
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== Biochemistry ==
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* [[Team:EPF-Lausanne/Protocols/IPTG test|IPTG test]]: test expression of a gene downstream from Plac
== Microfluidics ==
== Microfluidics ==
* [[Team:EPF-Lausanne/Protocols/PDMS_two_layer_device_fabrication|PDMS two layer device fabrication]]
* [[Team:EPF-Lausanne/Protocols/PDMS_two_layer_device_fabrication|PDMS two layer device fabrication]]
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* [[Team:EPF-Lausanne/Protocols/Master microfabrication for PDMS replica molding |Master microfabrication for PDMS replica molding]]
* [[Team:EPF-Lausanne/Protocols/MITOMI_protein_DNA|MITOMI: Protein – DNA interactions]]
* [[Team:EPF-Lausanne/Protocols/MITOMI_protein_DNA|MITOMI: Protein – DNA interactions]]
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* [[Team:EPF-Lausanne/Protocols/Klenow dsDNA synthesis|Klenow dsDNA synthesis]]
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== Worm culture ==
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* [[Team:EPF-Lausanne/Protocols/MG_Agar_Plates|Agar plate preparation]]: preparing MG Agar plates for worm cultures.
{{:Team:EPF-Lausanne/Templates/Footer}}
{{:Team:EPF-Lausanne/Templates/Footer}}

Latest revision as of 15:35, 16 October 2011