Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs

From 2011.igem.org

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==How==
==How==
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First we chose the <html> <a href="https://2011.igem.org/Team:EPF-Lausanne/Protocols/Site-specific_mutagenesis"> Site-specific mutagenesis </a></html> approach to make the mutants of TetR but that strategy <html> <a href="https://2011.igem.org/Team:EPF-Lausanne/Notebook/July2011#Tuesday.2C_12_July_2011"> was not working well </a> </html> and another one was tried in parallel: <html> <a href="https://2011.igem.org/Team:EPF-Lausanne/Protocols/TetR_Extension_PCR" PCR-induced mutagenesis.</a></html>  
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First we chose the <html> <a href="https://2011.igem.org/Team:EPF-Lausanne/Protocols/Site-specific_mutagenesis"> Site-specific mutagenesis </a></html> approach to make the mutants of TetR but that strategy <html> <a href="https://2011.igem.org/Team:EPF-Lausanne/Notebook/July2011#Tuesday.2C_12_July_2011"> was not working well </a> </html> and another one was tried in parallel: <html> <a href="https://2011.igem.org/Team:EPF-Lausanne/Protocols/TetR_Extension_PCR" PCR-induced mutagenesis.</a></html>
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Site-directed mutagenesis consists of amplifying plasmid and
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[[File:PCR_sketch.png|400px]]
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==What==
==What==

Revision as of 01:02, 22 September 2011