Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs

From 2011.igem.org

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{| {{table}} style="font-size: 60%;"
{| {{table}} style="font-size: 60%;"
| align="center" style="background:#f0f0f0;"|'''TetR variants'''
| align="center" style="background:#f0f0f0;"|'''TetR variants'''
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| align="center" style="background:#f0f0f0;"|'''Method'''
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| align="center" style="background:#f0f0f0;"|'''Mutagenesis'''
| align="center" style="background:#f0f0f0;"|'''Promoter'''
| align="center" style="background:#f0f0f0;"|'''Promoter'''
| align="center" style="background:#f0f0f0;"|'''C-terminal'''
| align="center" style="background:#f0f0f0;"|'''C-terminal'''
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|  <html> <a href="http://partsregistry.org/Part:BBa_K613013">V36F</a> </html> ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613013||||
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|  <html> <a href="http://partsregistry.org/Part:BBa_K613013">V36F</a> </html> ||site directed||SP6||eGFP||plasmid||worked well||v||BBa_K613013||||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613014"> V36FW43S</a> </html> ||PCR-induced mutagenesis||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014||||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613014"> V36FW43S</a> </html> ||PCR-induced||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014||||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613015"> E37AW43ST141A </a></html>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v
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| <html> <a href="http://partsregistry.org/Part:BBa_K613015"> E37AW43ST141A </a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v
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| <html> <a href="http://partsregistry.org/Part:BBa_K613016"> P39K </a> </html>||PCR-induced mutagenesis||T7||His-tag||template ||ITT tested and worked ok||v||BBa_K613016||v||v
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| <html> <a href="http://partsregistry.org/Part:BBa_K613016"> P39K </a> </html>||PCR-induced||T7||His-tag||template ||ITT tested and worked ok||v||BBa_K613016||v||v
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| <html> <a href="http://partsregistry.org/Part:BBa_K613017">Y42F</a></html>||PCR-induced mutagenesis||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v
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| <html> <a href="http://partsregistry.org/Part:BBa_K613017">Y42F</a></html>||PCR-induced||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v
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| <html> <a href="http://partsregistry.org/Part:BBa_K613018">Y42FK108E</a></html>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v
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| <html> <a href="http://partsregistry.org/Part:BBa_K613018">Y42FK108E</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v
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| <html> <a href="http://partsregistry.org/Part:BBa_K613019">P39QY42M</a> </html> ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613019||||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613019">P39QY42M</a> </html> ||site directed||SP6||eGFP||plasmid||worked well||v||BBa_K613019||||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613020">P39QY42ML197S</a></html>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613020||||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613020">P39QY42ML197S</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613020||||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613021">P39QY42ML52P</a></html>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613021||||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613021">P39QY42ML52P</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613021||||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613022">E37AP39K</a></html>||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613022||v||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613022">E37AP39K</a></html>||site directed||T7||His-tag||linear ||||||BBa_K613022||v||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613023">E37AP39QY42F</a></html>||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613023 ||v||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613023">E37AP39QY42F</a></html>||site directed||T7||His-tag||linear ||||||BBa_K613023 ||v||
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{{:Team:EPF-Lausanne/Templates/Footer}}
{{:Team:EPF-Lausanne/Templates/Footer}}

Revision as of 16:50, 21 September 2011

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