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Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs
From 2011.igem.org
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| align="center" style="background:#f0f0f0;"|'''TetR variants''' | | align="center" style="background:#f0f0f0;"|'''TetR variants''' | ||
| align="center" style="background:#f0f0f0;"|'''Method''' | | align="center" style="background:#f0f0f0;"|'''Method''' | ||
Revision as of 16:42, 21 September 2011
muTetRs
In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outlineWhy
How
What
| TetR variants | Method | Promoter | C-terminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
| V36F | site directed mutagenesis | SP6 | eGFP | plasmid | worked well | v | BBa_K613013 | ||
| V36FW43S | PCR-induced mutagenesis | T7 | His-tag | linear | (ins C-term), ITT tested | BBa_K613014 | |||
| E37AW43ST141A | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | v | BBa_K613015 | v | v |
| P39K | PCR-induced mutagenesis | T7 | His-tag | template | ITT tested and worked ok | v | BBa_K613016 | v | v |
| Y42F | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | v | BBa_K613017 | v | v |
| Y42FK108E | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | BBa_K613018 | v | v | |
| P39QY42M | site directed mutagenesis | SP6 | eGFP | plasmid | worked well | v | BBa_K613019 | ||
| P39QY42ML197S | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | BBa_K613020 | |||
| P39QY42ML52P | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | BBa_K613021 | |||
| E37AP39K | site directed mutagenesis | T7 | His-tag | linear | BBa_K613022 | v | |||
| E37AP39QY42F | site directed mutagenesis | T7 | His-tag | linear | BBa_K613023 | v |
