Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs

From 2011.igem.org

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==What==
==What==
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{| {{table}} style="font-size: 60%;"
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| align="center" style="background:#f0f0f0;"|'''TetR variants'''
| align="center" style="background:#f0f0f0;"|'''TetR variants'''
| align="center" style="background:#f0f0f0;"|'''Method'''
| align="center" style="background:#f0f0f0;"|'''Method'''
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| align="center" style="background:#f0f0f0;"|'''Sequenced'''
| align="center" style="background:#f0f0f0;"|'''Sequenced'''
| align="center" style="background:#f0f0f0;"|'''Sent to registry'''
| align="center" style="background:#f0f0f0;"|'''Sent to registry'''
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| align="center" style="background:#f0f0f0;"|''' in vivo test'''
 
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| V36F||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613013||||||might not bind ATC
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| V36F||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613013||||
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| V36FW43S||PCR-induced mutagenesis||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014||||||might not bind ATC
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| V36FW43S||PCR-induced mutagenesis||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014||||
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| E37AW43ST141A||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v||might not bind ATC
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| E37AW43ST141A||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v
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| P39K||PCR-induced mutagenesis||T7||His-tag||template ||ITT tested and worked ok||v||BBa_K613016||v||v||failed (Gibson?) will be sequenced
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| P39K||PCR-induced mutagenesis||T7||His-tag||template ||ITT tested and worked ok||v||BBa_K613016||v||v
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| Y42F||PCR-induced mutagenesis||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v||Gibson failed
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| Y42F||PCR-induced mutagenesis||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v
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| Y42FK108E||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v||behaves as WT
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| Y42FK108E||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v
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| P39QY42M  ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613019||||||failed (Gibson?) will be sequenced
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| P39QY42M  ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613019||||
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| P39QY42ML197S||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613020||||||
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| P39QY42ML197S||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613020||||
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| P39QY42ML52P||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613021||||||
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| P39QY42ML52P||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613021||||
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| E37AP39K||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613022||v||||
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| E37AP39K||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613022||v||
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| E37AP39QY42F||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613023 ||v||||
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| E37AP39QY42F||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613023 ||v||
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{{:Team:EPF-Lausanne/Templates/Footer}}
{{:Team:EPF-Lausanne/Templates/Footer}}

Revision as of 16:42, 21 September 2011

Retrieved from "http://2011.igem.org/Team:EPF-Lausanne/Our_Project/TetR_mutants/muTetRs"