Team:EPF-Lausanne/Our Project/T7 promoter variants/recovery

From 2011.igem.org

(Difference between revisions)
(Results)
Line 21: Line 21:
=== Results ===
=== Results ===
-
[[File:first_dnarecov_OD.png|450px|left]]
+
[[File:first_dnarecov_OD.png|450px]]
Optical density measurements were made every hour for 12 hours. The non-lysing cell culture grew as expected into a cloudy mix of cells, while the lysing cell culture showed ever-decreasing optical density. This graph confirms that lysis was indeed taking place.  
Optical density measurements were made every hour for 12 hours. The non-lysing cell culture grew as expected into a cloudy mix of cells, while the lysing cell culture showed ever-decreasing optical density. This graph confirms that lysis was indeed taking place.  
-
[[File:first_DNA_supernatant.png|450px|left]]
+
[[File:first_DNA_supernatant.png|450px]]
This chart shows the concentration of mRFP genes in the supernatant for each culture as the result of a qPCR. Both cultures contained cells with RFP plasmids. The lysing culture shows a definite increase in the number of mRFP plasmids in the supernatant over the course of the 10 hour experiment. The non-lysing culture shows a quasi-constant number of plasmids available in the supernatant. We can conclude that the DNA from the relevant lysed cells can be recovered from the supernatant.
This chart shows the concentration of mRFP genes in the supernatant for each culture as the result of a qPCR. Both cultures contained cells with RFP plasmids. The lysing culture shows a definite increase in the number of mRFP plasmids in the supernatant over the course of the 10 hour experiment. The non-lysing culture shows a quasi-constant number of plasmids available in the supernatant. We can conclude that the DNA from the relevant lysed cells can be recovered from the supernatant.
-
[[File:firstCFU.png|450px|left]]
+
[[File:firstCFU.png|450px]]
The colony fluorescence, the second method for quantifying the amount of recovered DNA, relates the number of colonies to the amount of transformant (in uL) that was plated. We find that the number of colonies taken from the lysing culture (all pink because of RFP) increases dramatically over time, while the number of colonies from the non-lysing culture is near-zero over the 10 hours of the experiment. This result is an additional confirmation that DNA was recovered.  
The colony fluorescence, the second method for quantifying the amount of recovered DNA, relates the number of colonies to the amount of transformant (in uL) that was plated. We find that the number of colonies taken from the lysing culture (all pink because of RFP) increases dramatically over time, while the number of colonies from the non-lysing culture is near-zero over the 10 hours of the experiment. This result is an additional confirmation that DNA was recovered.  
{{:Team:EPF-Lausanne/Templates/Footer}}
{{:Team:EPF-Lausanne/Templates/Footer}}

Revision as of 15:16, 21 September 2011