Team:EPF-Lausanne/Our Project/Data
From 2011.igem.org
Data
team, please check this page: [1]
Contents |
New parts
we must have 3 favourite, then the others come in a "other biobrick" section
Variants without additional lac operator (constitutive):
Variants with additional lac operator (LacI repressed):
Medium-strength Plac
blablabla
TetR Mutants
TetR variants | Mutagenesis | Promoter | Cterminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
---|---|---|---|---|---|---|---|---|---|
E37AW43ST141A | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613015 | v | v |
P39K | PCR-induced | T7 | His-tag | template | ITT tested, worked | v | BBa_K613016 | v | v |
Y42F | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613017 | v | v |
Y42FK108E | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613018 | v | v |
T7 promoter variants
Pre-existing parts
add something about the lysis cassette, and add also on its page on the partsregistry!
In vivo characterization - Readout systems
We created two different readout systems to characterize TetR mutants in vivo. This is the last step of our whole method, after having selected the interesting TetR-Ptet mutant pairs with the lysis device and having characterized them in vitro.
TetR - RFP
The first readout system is composed of TetR with a constitutive promoter, followed by RFP with a Ptet promoter. If TetR binds to Ptet, then RFP is repressed. For more details about them and the experimental results, please refer to the Reporter systems page.
TetR - LacI - RFP
This second readout system is composed of 3 genes: TetR under Pconst control, LacI under Ptet control (playing the role of an inverter) and finally RFP under pLac control. Here, RFP is induced when TetR binds to pTet. The experimental results are available on the Reporter systems page.