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Team:EPF-Lausanne/Our Project/Data
From 2011.igem.org
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== Other New parts == | == Other New parts == | ||
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| + | === T7 Promoter === | ||
Our lysis selection device uses what we refer to as the wild-type T7 Promoter: | Our lysis selection device uses what we refer to as the wild-type T7 Promoter: | ||
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[http://partsregistry.org/Part:BBa_K613012 K613012] | [http://partsregistry.org/Part:BBa_K613012 K613012] | ||
| + | === TetR mutants === | ||
In-vivo testing was used to characterise these: | In-vivo testing was used to characterise these: | ||
Revision as of 01:54, 22 September 2011
Data
team, please check this page: [1]
Contents |
Favourite Parts
These are officially our three favourite parts:
Other New parts
T7 Promoter
Our lysis selection device uses what we refer to as the wild-type T7 Promoter:
In addition, we submitted the following mutants:
Variants without additional lac operator (constitutive):
Variants with additional lac operator (LacI repressed):
TetR mutants
In-vivo testing was used to characterise these:
- V36F mutant: K613013
- V36F W43S double mutant: K613014
- E37A W43S T141A triple mutant: K613015
- P39K mutant: K613016
- Y42F K108E double mutant: K613018
- P39Q Y42M double mutant: K613019
Medium-strength Plac
TetR Mutants
| TetR variants | Mutagenesis | Promoter | Cterminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
|---|---|---|---|---|---|---|---|---|---|
| E37AW43ST141A | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613015 | v | v |
| P39K | PCR-induced | T7 | His-tag | template | ITT tested, worked | v | BBa_K613016 | v | v |
| Y42F | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613017 | v | v |
| Y42FK108E | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613018 | v | v |
T7 promoter variants
Pre-existing parts we improved
- Experience - The Berkeley Lysis Device, BBa_K112808: In developing our Lysis selection device, we characterised induction, and ran proof-of-principle experiments.
