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Team:EPF-Lausanne/Our Project/Data
From 2011.igem.org
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== Pre-existing parts we improved == | == Pre-existing parts we improved == | ||
| - | - [http://partsregistry.org/Part:BBa_K112808:Experience Experience] - | + | - [http://partsregistry.org/Part:BBa_K112808:Experience Experience] - '''The Berkeley Lysis Device''' BBa_K112808: In developing our Lysis selection device, we characterised induction, and ran proof-of-principle experiments. |
{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} | ||
Revision as of 01:28, 22 September 2011
Data
team, please check this page: [1]
Contents |
New parts
we must have 3 favourite, then the others come in a "other biobrick" section
Variants without additional lac operator (constitutive):
Variants with additional lac operator (LacI repressed):
Medium-strength Plac
TetR Mutants
| TetR variants | Mutagenesis | Promoter | Cterminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
|---|---|---|---|---|---|---|---|---|---|
| E37AW43ST141A | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613015 | v | v |
| P39K | PCR-induced | T7 | His-tag | template | ITT tested, worked | v | BBa_K613016 | v | v |
| Y42F | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613017 | v | v |
| Y42FK108E | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613018 | v | v |
T7 promoter variants
Pre-existing parts we improved
- Experience - The Berkeley Lysis Device BBa_K112808: In developing our Lysis selection device, we characterised induction, and ran proof-of-principle experiments.
