Revision as of 01:21, 22 September 2011

Data

team, please check this page: [1]

we must have 3 favourite, then the others come in a "other biobrick" section

Variants without additional lac operator (constitutive):

Variants with additional lac operator (LacI repressed):

blablabla

add something about the lysis cassette, and add also on its page on the partsregistry!

@@ Line 82: / Line 82: @@
   add something about the lysis cassette, and add also on its page on the partsregistry!
-== In vivo characterization - Readout systems ==
-We created two different readout systems to characterize TetR mutants ''in vivo''. This is the last step of our whole method, after having selected the interesting TetR-Ptet mutant pairs with the lysis device and having characterized them ''in vitro''.
-=== TetR - RFP ===
-The first readout system is composed of TetR with a constitutive promoter, followed by RFP with a Ptet promoter. If TetR '''binds''' to Ptet, then RFP is ''' repressed'''. For more details about them and the experimental results, please refer to the [https://2011.igem.org/Team:EPF-Lausanne/Our_Project/Assembly#TetR_-_RFP_system Reporter systems] page.
-[[File:EPFL_2reporters_ss.JPG|400px]]
-=== TetR - LacI - RFP ===
-This second readout system is composed of 3 genes: TetR under Pconst control, LacI under Ptet control (playing the role of an inverter) and finally RFP under pLac control. Here, RFP is '''induced''' when TetR '''binds''' to pTet. The experimental results are available on the [https://2011.igem.org/Team:EPF-Lausanne/Our_Project/Assembly#TetR_-_LacI_-_RFP_system Reporter systems] page.
-[[File:EPFL_3reporter_ss.JPG|400px]]
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