Team:EPF-Lausanne/Our Project/Assembly/Plac

From 2011.igem.org

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[[File:EPFL_Nadine-Plac-induction.png|600px]]
[[File:EPFL_Nadine-Plac-induction.png|600px]]
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The cells treated with IPTG produced more RFP than their non-treated counterparts; this different is however not striking, showing that the basal expression of LacI by our DH5alpha cells is quite low. The normal expression of RFP driven by our Plac promoter is of 530 normalized RFUs; the highest value is 640 normalized RFUs.
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The cells treated with IPTG produced more RFP than their non-treated counterparts; this different is however not striking, showing that the basal expression of LacI by our DH5alpha cells is quite low. The normal expression of RFP driven by our Plac promoter is of 530 normalized RFUs; the highest value is 640 normalized RFUs.  
''Dose-response curve''
''Dose-response curve''
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[[File:EPFL_Nadine-Plac-doseresponse.png|600px]]
[[File:EPFL_Nadine-Plac-doseresponse.png|600px]]
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This graphs shows the action of IPTG on RFU output. Since it doesn't seem to saturate, there was perhaps still some repression by LacI in our cells.
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This graphs shows the action of IPTG on RFU output. Since it doesn't seem to saturate, there was perhaps still some repression by LacI going on in our cells. Interestingly, LacI does have a quantifiable action on our Ptet promoter - meaning that we can use it efficiently in our second [https://2011.igem.org/Team:EPF-Lausanne/Our_Project/Assembly#TetR_-_LacI_-_RFP_system reporter system].
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Revision as of 18:40, 20 September 2011