Team:EPF-Lausanne/Notebook/May2011

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Note: this is not week 1. We are just writing here to have it somewhere
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== Tuesday, 10 May 2011 ==
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Clara and Henrike did the TetR linear template
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Results:
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Gene PCR and extension PCR
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[[File:PCR1 inverted.png|500px]]
== Wednesday, 11 May 2011 ==
== Wednesday, 11 May 2011 ==
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==Thursday, 26 May 2011==
==Thursday, 26 May 2011==
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Competance of the prepared DH5α T1res was measured, 10^5 of CFU/µg . (Henrike, Clara, Lilia).  
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Competance of the prepared DH5α T1res was measured, 10^5 of CFU/µg (Henrike, Clara, Lilia).  
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λ and T4 lysis device plasmids were transformed, but only T4 lysis device transformation gave 3 colonies, nothing for λ samples.
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λ and T4 lysis device plasmids were transformed, but only T4 lysis device transformation gave 3 colonies, nothing for λ samples.
== Friday, 27 May 2011 ==
== Friday, 27 May 2011 ==
Using Invitrogen PureLink Quick miniprep kit, plasmids containing TetR-GFP, LacI and pSB1A2 plasmids with T4LysisDevice were purified. For TetR-GFP  162ng/µl concentration was obtained and for other samples ~172ng/µl. (Lilia)
Using Invitrogen PureLink Quick miniprep kit, plasmids containing TetR-GFP, LacI and pSB1A2 plasmids with T4LysisDevice were purified. For TetR-GFP  162ng/µl concentration was obtained and for other samples ~172ng/µl. (Lilia)
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== Tuesday, 31 May 2011 ==
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Mixed primers to sequence verify the lysis device. Mixed the seven primers designed on 13 May to plasmids containing the T4 Lysis Device, biobrick K112808 (extracted 27 May). For each primer, prepared a 10 ul solution containing 20 pmol primer and 865.5 g plasmid.
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Sent to microsynth for sequencing the following day. (Douglas)
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Latest revision as of 16:49, 5 July 2011