Team:EPF-Lausanne/Notebook/May2011

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Note: this is not week 1. We are just writing here to have it somewhere
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== Tuesday, 10 May 2011 ==
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Clara and Henrike did the TetR linear template
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Results:
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Gene PCR and extension PCR
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[[File:PCR1 inverted.png|500px]]
== Wednesday, 11 May 2011 ==
== Wednesday, 11 May 2011 ==
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* Developed in PGMEA. The three non-baked wafers are now stripped and blank again.
* Developed in PGMEA. The three non-baked wafers are now stripped and blank again.
* Valid wafer verified by optical microscopy and contact profilometry. Some dust contamination, '''will need to be blow-cleaned before PDMS moulding'''.
* Valid wafer verified by optical microscopy and contact profilometry. Some dust contamination, '''will need to be blow-cleaned before PDMS moulding'''.
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* The '''three other wafers can be cleaned and re-used''' (SRD, Pirana solution, 02 plasma strip).
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* The '''three other wafers can be cleaned and re-used''' (SRD, Piranha solution, 02 plasma strip).
For details of the process, see protocol for SU8 processes: [http://cmi.epfl.ch/photo/photo_process/files/Sawatec_processSU8.php| SU8 Process by CMI]
For details of the process, see protocol for SU8 processes: [http://cmi.epfl.ch/photo/photo_process/files/Sawatec_processSU8.php| SU8 Process by CMI]
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Alina and Douglas prepared solutions of LacI and primers for sequencing, ready to send out to microsynth.
Alina and Douglas prepared solutions of LacI and primers for sequencing, ready to send out to microsynth.
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== Friday, 20 May 2011 ==
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Competant cells (DH5α T1 resistant) were prepared by Henrike, Lilia and Clara using Henrike's protocol, they are stored in a box at -80°C, location indicated on the door of the fridge.
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==Thursday, 26 May 2011==
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Competance of the prepared DH5α T1res was measured, 10^5 of CFU/µg (Henrike, Clara, Lilia).
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λ and T4 lysis device plasmids were transformed, but only T4 lysis device transformation gave 3 colonies, nothing for λ samples.
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== Friday, 27 May 2011 ==
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Using Invitrogen PureLink Quick miniprep kit, plasmids containing TetR-GFP, LacI and pSB1A2 plasmids with T4LysisDevice were purified. For TetR-GFP  162ng/µl concentration was obtained and for other samples ~172ng/µl. (Lilia)
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== Tuesday, 31 May 2011 ==
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Mixed primers to sequence verify the lysis device. Mixed the seven primers designed on 13 May to plasmids containing the T4 Lysis Device, biobrick K112808 (extracted 27 May). For each primer, prepared a 10 ul solution containing 20 pmol primer and 865.5 g plasmid.
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Sent to microsynth for sequencing the following day. (Douglas)
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Latest revision as of 16:49, 5 July 2011