Team:EPF-Lausanne/Notebook/July2011

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(Friday, 29 July 2011)
(Friday, 29 July 2011)
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Douglas and Alessandro's transformed cells didn't grow (maybe problems with competent cells?).
Douglas and Alessandro's transformed cells didn't grow (maybe problems with competent cells?).
Alessandro transformed the cells again (with the J61002-Ptet-RFP Gibson plasmid) but this time he trasformed also the sample labeled Col1-RFP and Col4-RFP (supposing that these are the same as J61002-Ptet-RFP Gibson plasmid).
Alessandro transformed the cells again (with the J61002-Ptet-RFP Gibson plasmid) but this time he trasformed also the sample labeled Col1-RFP and Col4-RFP (supposing that these are the same as J61002-Ptet-RFP Gibson plasmid).
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Alessandro transformed the Gibson assembly made by Douglas on Lilia's (or Alina's?) competent cells and also on Henrike's competent cells. Due to an error only the lysis cassette has been transformed. On Tuesday the Gibson assembly has to be repeated since we ran out of the previous one.
The Mutation PCR yielded no product, probably because the primers were too diluted. It has been repeated with a higher primer concentration.
The Mutation PCR yielded no product, probably because the primers were too diluted. It has been repeated with a higher primer concentration.
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Revision as of 15:24, 29 July 2011