Team:Cornell/Lysis

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<div style="padding-top:5px; border-bottom:solid">
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<table style="margin-left:auto; margin-right:auto;width:93%;padding-top: 10px;">
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<a href="https://2011.igem.org/Team:Cornell/Modo">
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<img style="width: 175px; height: 135px;" alt="alttext" src=" http://i1208.photobucket.com/albums/cc378/CornelliGEM/azdgdrFinalColorOutput.png">
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</a>
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</div>
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</td>
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<div>
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<a href="https://2011.igem.org/Team:Cornell/Video">
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<img style="width: 175px; height: 135px;" alt="alttext" src=" http://i1208.photobucket.com/albums/cc378/CornelliGEM/PosterImageFinalColorOutput-1.png">
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</div>
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</td>
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<td>
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<div>
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<a href="https://2011.igem.org/Team:Cornell/Construction">
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<img style="width: 175px; height: 135px;" alt="alttext" src="http://i1208.photobucket.com/albums/cc378/CornelliGEM/BiggerandBetterFinalColorOutput.png">
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</a>
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</div>
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</td>
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<td>
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<div>
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<a href="https://2011.igem.org/Team:Cornell/Trailer">
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<img style="width: 175px; height: 135px;" alt="alttext" src="http://i1208.photobucket.com/albums/cc378/CornelliGEM/biofactoryFinalColorOutput.png">
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</a>
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</div>
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</td>
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<td>
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<div>
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<a href="https://2011.igem.org/Team:Cornell/Modo">
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<img style="width: 175px; height: 135px;" alt="alttext" src="http://i1208.photobucket.com/albums/cc378/CornelliGEM/biofactoryFinalColorOutput.png">
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</a>
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</div>
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</td>
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</tr>
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</table>
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<div style="padding-top:10px; border-bottom:solid">
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</div>
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<div style = "
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margin-left:auto;
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margin-right:auto;
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width:93%;
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background.image:(http://i1208.photobucket.com/albums/cc378/CornelliGEM/Final10146FinalColorOutput.png); position:relative;margin-left:auto; margin-right:auto; padding-top:25px;">
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<img src="http://i1208.photobucket.com/albums/cc378/CornelliGEM/Final10146FinalColorOutput.png" border="0" alt="Photobucket">
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<div style="font-family:Arial;font-size:20pt; font-weight:bold; padding-top:25px;margin-left:auto;margin-right:auto;width:93%;">
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Lysis Scene
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</div>
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<div style ="background-color: white;width:900px; border-style:solid; border-width:0px; border-color:rgb(215, 205, 159); margin-left:auto; margin-right:auto; width:93%; padding-top: 0px">
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<p style="margin:0in;font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt">&nbsp;</p>
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<p style="margin:0in;margin-left:.375in;font-family:&quot;Comic Sans MS&quot;;font-size:
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10.0pt"><span style="font-weight:bold">Original Description:</span></p>
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<ul type="disc" style="margin-left:.75in;direction:ltr;unicode-bidi:embed;
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margin-top:0in;margin-bottom:0in">
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<li style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style:italic;font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt">Beaker
 +
    of cells floating. Zoom into cells. Watch them float around<span>&nbsp; </span>briefly and zoom close up to 1. Show
 +
    Green Beams of Light"</span></li>
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</ul>
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<ul type="disc" style="margin-left:.75in;direction:ltr;unicode-bidi:embed;
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margin-top:0in;margin-bottom:0in">
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<li style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style:italic;font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt">Zoom
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    out to edge of cell. Small rod-shaped surface proteins pepper<span>&nbsp; </span>surface then aggregate into rings. Cell
 +
    bulges, inflates, and bursts<span>&nbsp; </span>like a
 +
    balloon.</span></li>
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</ul>
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<ul type="disc" style="margin-left:.75in;direction:ltr;unicode-bidi:embed;
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margin-top:0in;margin-bottom:0in">
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<li style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style:italic;font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt">Zoom
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    out as multiple cells burst. </span></li>
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</ul>
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<p style="margin:0in;margin-left:.375in;font-family:&quot;Comic Sans MS&quot;;font-size:
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10.0pt">&nbsp;</p>
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<p style="margin:0in;margin-left:.375in;font-family:&quot;Comic Sans MS&quot;;font-size:
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10.0pt"><span style="font-weight:bold">Interpreted Components:</span></p>
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 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
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<li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">E. Coli
 +
    Model</span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Cell Wall
 +
      - needs to be able to disintegrate.</span></li>
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</ol>
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<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="2" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Protoplasm
 +
      - needs maintain shape within cell wall.</span></li>
 +
</ol>
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</ol>
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 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="2" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Surface
 +
    Protein Coating</span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Surface
 +
      Proteins - appearance.</span></li>
 +
</ol>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="2" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Coating
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      Pattern</span></li>
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</ol>
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</ol>
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<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="3" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Explosion
 +
    Technique</span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Bursting
 +
      Effect.</span></li>
 +
</ol>
 +
</ol>
 +
 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="4" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Light
 +
    Source</span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Show
 +
      green color and purpose without being too intrusive.</span></li>
 +
</ol>
 +
</ol>
 +
 +
<p style="margin:0in;margin-left:.75in;font-family:&quot;Comic Sans MS&quot;;font-size:
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10.0pt">&nbsp;</p>
 +
 +
<p style="margin:0in;margin-left:.375in;font-family:&quot;Comic Sans MS&quot;;font-size:
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10.0pt"><span style="font-weight:bold">Components Development Summary</span></p>
 +
 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Cell Wall </span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Created
 +
      Mesh of E. coli. Sculpted it with a mesh of protoplasm underneath to
 +
      maintain consistency in cell wall spacing. Sculpted morph maps to show
 +
      wall "gaining holes." At this point in time, skills were very
 +
      limited. No viable nice way to explode this.</span></li>
 +
</ol>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="2" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Replicator
 +
      Coating - an actual cell wall. Designed by coating the protoplasm mesh
 +
      with thousands of individual "platelet" meshes. With a surface
 +
      generator and vertex map deformers, was able to manipulate it very
 +
      nicely. Individual platelets also helped give E. coli more life.</span></li>
 +
</ol>
 +
</ol>
 +
 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="2" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Protoplasm</span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Created
 +
      mesh of protoplasm underneath cell wall. Worked on appearance. Team
 +
      leader requested "wispy" protoplasm. Although cell wall mesh
 +
      was scratched, protoplasm mesh was very usable and underwent vertex map
 +
      deformation in sync with its replicator cell wall shell.</span></li>
 +
</ol>
 +
</ol>
 +
 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="3" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Surface
 +
    Proteins</span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Modeled a
 +
      stand-in protein model in modo. Textured with a modified version of Yazan
 +
      Malkosh's electron microscope texture. Team liked appearance. </span></li>
 +
</ol>
 +
</ol>
 +
 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="4" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Coating
 +
    Pattern</span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Used
 +
      vertex map deformers. Took several attempts to get right.</span></li>
 +
</ol>
 +
</ol>
 +
 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="5" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Explosion
 +
    Technique:</span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Experimented
 +
      with "gooey" and "hard" explosions for meshes. Not
 +
      satisfied. </span></li>
 +
</ol>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="2" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Experimented
 +
      with dynamics package. Far too resource intensive. Decided replicator
 +
      techniques would be more effective.</span></li>
 +
</ol>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="3" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Resultantly
 +
      used a combination of vertex map deformers and morph maps on all
 +
      replicators and meshes. Several renditions --&gt; Settled on "pop
 +
      and expand" rendition.</span></li>
 +
</ol>
 +
</ol>
 +
 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="6" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Beams of
 +
    Light:</span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Started
 +
      using volumetric<span>&nbsp; </span>direction light
 +
      sources. Didn't quite like the result. Experimented. Settled on
 +
      volumetric point light source with light shift over time. Also gave the
 +
      scene an effective "Sun" </span></li>
 +
</ol>
 +
</ol>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">&nbsp;</p>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">&nbsp;</p>
 +
 +
<p style="margin:0in;margin-left:.375in;font-family:&quot;Comic Sans MS&quot;;font-size:
 +
10.0pt"><span style="font-weight:bold">Summary of Scene Progression</span></p>
 +
 +
<p style="margin:0in;margin-left:.375in;font-family:&quot;Comic Sans MS&quot;;font-size:
 +
10.0pt"><span style="font-style:italic">For the lysis scene, Modo skill set was
 +
still being developed. As a result, scene involved a good amount of trial and
 +
error with various scene rewrites.</span></p>
 +
 +
<ol type="1" style="margin-left:.75in;direction:ltr;unicode-bidi:embed;
 +
margin-top:0in;margin-bottom:0in;font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;
 +
font-weight:bold;font-style:normal">
 +
<li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle;
 +
    font-weight:bold"><span style="font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;
 +
    font-weight:bold;font-style:normal;font-weight:bold;font-family:&quot;Comic Sans MS&quot;;
 +
    font-size:10.0pt">Pure Mesh Attempt.(</span><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">06/26/2011 - 07/03/2011</span><span style="font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;font-weight:bold;
 +
    font-style:normal;font-weight:bold;font-family:&quot;Comic Sans MS&quot;;font-size:
 +
    10.0pt">)</span></li>
 +
</ol>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt"><span style="font-weight:bold"><span>&nbsp;</span></span>Looked very nice in stills. Scratched
 +
before significantly animated.</p>
 +
 +
<ol type="1" style="margin-left:.75in;direction:ltr;unicode-bidi:embed;
 +
margin-top:0in;margin-bottom:0in;font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;
 +
font-weight:bold;font-style:normal">
 +
<li value="2" style="margin-top:0;margin-bottom:0;vertical-align:middle;
 +
    font-weight:bold"><span style="font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;
 +
    font-weight:bold;font-style:normal;font-weight:bold;font-family:&quot;Comic Sans MS&quot;;
 +
    font-size:10.0pt">1st Draft - Concept visualization. (</span><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">07/03/2011 - 07/10/2011)</span></li>
 +
</ol>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">Created two animation models for the major elements of the
 +
scene.</p>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">The first illustrated replicator offset explosions with<span>&nbsp; </span>holes in the cell wall. Positive Reception.</p>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">The second showcased surface protein patterns and
 +
deformations. Needed some critiquing - rings vs. globs. </p>
 +
 +
<ol type="1" style="margin-left:.75in;direction:ltr;unicode-bidi:embed;
 +
margin-top:0in;margin-bottom:0in;font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;
 +
font-weight:bold;font-style:normal">
 +
<li value="3" style="margin-top:0;margin-bottom:0;vertical-align:middle;
 +
    font-weight:bold"><span style="font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;
 +
    font-weight:bold;font-style:normal;font-weight:bold;font-family:&quot;Comic Sans MS&quot;;
 +
    font-size:10.0pt">2nd Draft - Flagella, Scale Up + Replicator explosion. (</span><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">07/10/2011 - 07/11/2011</span><span style="font-family:
 +
    &quot;Comic Sans MS&quot;;font-size:10.0pt;font-weight:bold;font-style:normal;
 +
    font-weight:bold;font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt">)</span></li>
 +
</ol>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">Resulted from an experiment with flagella. Protoplasm mesh is
 +
coated with a cell wall replicator that does not deform. Scaling up the
 +
protoplasm resulted in the expansion and "disintegration" of the cell
 +
wall. Flagella was dynamically parented to protoplasm for smooth motion weight
 +
gradient. Met with positive reception, but team decided to cut flagella for
 +
next rendition0. Rendition also did not include surface proteins.</p>
 +
 +
<ol type="1" style="margin-left:.75in;direction:ltr;unicode-bidi:embed;
 +
margin-top:0in;margin-bottom:0in;font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;
 +
font-weight:bold;font-style:normal">
 +
<li value="4" style="margin-top:0;margin-bottom:0;vertical-align:middle;
 +
    font-weight:bold"><span style="font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;
 +
    font-weight:bold;font-style:normal;font-weight:bold;font-family:&quot;Comic Sans MS&quot;;
 +
    font-size:10.0pt">3rd Draft - 1st Full Assembly. (</span><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">07/11/2011 - 07/15/2011</span><span style="font-family:
 +
    &quot;Comic Sans MS&quot;;font-size:10.0pt;font-weight:bold;font-style:normal;
 +
    font-weight:bold;font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt">)</span></li>
 +
</ol>
 +
 +
<p style="margin:0in;margin-left:.75in;font-family:&quot;Comic Sans MS&quot;;font-size:
 +
10.0pt">Rebuilt scene using feedback and lessons from previous renditions. </p>
 +
 +
<ol type="i" style="margin-left: 1.7812in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Visual
 +
    Overhaul. Used edited version of Yazan Malkosh's electron microscope
 +
    texture for cell wall platelets</span></li>
 +
</ol>
 +
 +
<ol type="i" style="margin-left: 1.7812in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="2" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Updated
 +
    and refined vertex map deformers.</span></li>
 +
</ol>
 +
 +
<ol type="i" style="margin-left: 1.7812in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="3" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Refined
 +
    explosion to include organic scaling of bacteria and post-explosion
 +
    swirling of cell wall platelets and expansion of protoplasm.</span></li>
 +
</ol>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">&nbsp;</p>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">Mostly positive reception. Team leader wanted more prominent
 +
"spikey" deformations at holes, and more of a burst explosion.
 +
Consensus<span>&nbsp; </span>- camera work made bacteria
 +
appear to shrink. Also needed to implement surface protein layers.</p>
 +
 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="5" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;font-weight:bold;
 +
    font-style:normal;font-weight:bold;font-family:&quot;Comic Sans MS&quot;;font-size:
 +
    10.0pt">4th Draft - 2nd Full Assembly (</span><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">07/15/2011 - 07/20/2011</span><span style="font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;font-weight:bold;
 +
    font-style:normal;font-weight:bold;font-family:&quot;Comic Sans MS&quot;;font-size:
 +
    10.0pt">)</span></li>
 +
</ol>
 +
 +
<p style="margin:0in;margin-left:.75in;font-family:&quot;Comic Sans MS&quot;;font-size:
 +
10.0pt">Refined scene</p>
 +
 +
<ol type="i" style="margin-left: 1.7812in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Rebuilt
 +
    and implemented surface protein vertex map deformers. Protein visuals
 +
    updated.</span></li>
 +
</ol>
 +
 +
<ol type="i" style="margin-left: 1.7812in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="2" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Implemented
 +
    changes to hole deformations. More of a spikey look.</span></li>
 +
</ol>
 +
 +
<ol type="i" style="margin-left: 1.7812in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="3" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Modified
 +
    camera work.</span></li>
 +
</ol>
 +
 +
<ol type="i" style="margin-left: 1.7812in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="4" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Refined
 +
    Explosion - more of a solid pop and burst.</span></li>
 +
</ol>
 +
 +
<ol type="i" style="margin-left: 1.7812in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="5" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Updated
 +
    green light source</span></li>
 +
</ol>
 +
 +
<ol type="i" style="margin-left: 1.7812in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="6" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Changed
 +
    protoplasm to a less resource intensive material.</span></li>
 +
</ol>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">&nbsp;</p>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">Positive reception. Results most satisfactory. Comments that
 +
holes were too spikey.</p>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">At present, will correct if have time</p>
 +
 +
<ol type="1" style="margin-left: 0.75in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
<li value="6" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-family:&quot;Comic Sans MS&quot;;font-size:10.0pt;font-weight:bold;
 +
    font-style:normal;font-weight:bold;font-family:&quot;Comic Sans MS&quot;;font-size:
 +
    10.0pt">Experimental Methods (</span><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">08/08/2011 - present)</span></li>
 +
<ol type="i" style="margin-left: 1.0312in; direction: ltr; unicode-bidi: embed; margin-top: 0in; margin-bottom: 0in; font-family: 'Comic Sans MS'; font-size: 10pt; font-style: normal; ">
 +
  <li value="1" style="margin-top:0;margin-bottom:0;vertical-align:middle"><span style="font-style: normal; font-family: 'Comic Sans MS'; font-size: 10pt; ">Never
 +
      implemented in-scene. Post-2nd full assembly,<span>&nbsp; </span>developed a technique for nicer cell
 +
      wall disintegration around holes. Technique would involve a complete
 +
      rebuild. If time, plan to implement.</span></li>
 +
</ol>
 +
</ol>
 +
 +
<p style="margin:0in;margin-left:1.7812in;font-family:&quot;Comic Sans MS&quot;;
 +
font-size:10.0pt">&nbsp;</p>
 +
 +
<p style="margin:0in;margin-left:.375in;font-family:&quot;Comic Sans MS&quot;;font-size:
 +
10.0pt"><span style="font-weight:bold">Reception and Review: </span>Satisfactory.
 +
Potentially scene could use some slight improvements - namely
 +
"spikes" and end protoplasm appearance. Otherwise, current result is
 +
nicely polished.</p>
 +
 +
</div>
 +
</div>
 +
 +
 +
</html>

Revision as of 10:53, 28 September 2011

Photobucket
Lysis Scene

 

Original Description:

  • Beaker of cells floating. Zoom into cells. Watch them float around  briefly and zoom close up to 1. Show Green Beams of Light"
  • Zoom out to edge of cell. Small rod-shaped surface proteins pepper  surface then aggregate into rings. Cell bulges, inflates, and bursts  like a balloon.
  • Zoom out as multiple cells burst.

 

Interpreted Components:

  1. E. Coli Model
    1. Cell Wall - needs to be able to disintegrate.
    1. Protoplasm - needs maintain shape within cell wall.
  1. Surface Protein Coating
    1. Surface Proteins - appearance.
    1. Coating Pattern
  1. Explosion Technique
    1. Bursting Effect.
  1. Light Source
    1. Show green color and purpose without being too intrusive.

 

Components Development Summary

  1. Cell Wall
    1. Created Mesh of E. coli. Sculpted it with a mesh of protoplasm underneath to maintain consistency in cell wall spacing. Sculpted morph maps to show wall "gaining holes." At this point in time, skills were very limited. No viable nice way to explode this.
    1. Replicator Coating - an actual cell wall. Designed by coating the protoplasm mesh with thousands of individual "platelet" meshes. With a surface generator and vertex map deformers, was able to manipulate it very nicely. Individual platelets also helped give E. coli more life.
  1. Protoplasm
    1. Created mesh of protoplasm underneath cell wall. Worked on appearance. Team leader requested "wispy" protoplasm. Although cell wall mesh was scratched, protoplasm mesh was very usable and underwent vertex map deformation in sync with its replicator cell wall shell.
  1. Surface Proteins
    1. Modeled a stand-in protein model in modo. Textured with a modified version of Yazan Malkosh's electron microscope texture. Team liked appearance.
  1. Coating Pattern
    1. Used vertex map deformers. Took several attempts to get right.
  1. Explosion Technique:
    1. Experimented with "gooey" and "hard" explosions for meshes. Not satisfied.
    1. Experimented with dynamics package. Far too resource intensive. Decided replicator techniques would be more effective.
    1. Resultantly used a combination of vertex map deformers and morph maps on all replicators and meshes. Several renditions --> Settled on "pop and expand" rendition.
  1. Beams of Light:
    1. Started using volumetric  direction light sources. Didn't quite like the result. Experimented. Settled on volumetric point light source with light shift over time. Also gave the scene an effective "Sun"

 

 

Summary of Scene Progression

For the lysis scene, Modo skill set was still being developed. As a result, scene involved a good amount of trial and error with various scene rewrites.

  1. Pure Mesh Attempt.(06/26/2011 - 07/03/2011)

 Looked very nice in stills. Scratched before significantly animated.

  1. 1st Draft - Concept visualization. (07/03/2011 - 07/10/2011)

Created two animation models for the major elements of the scene.

The first illustrated replicator offset explosions with  holes in the cell wall. Positive Reception.

The second showcased surface protein patterns and deformations. Needed some critiquing - rings vs. globs.

  1. 2nd Draft - Flagella, Scale Up + Replicator explosion. (07/10/2011 - 07/11/2011)

Resulted from an experiment with flagella. Protoplasm mesh is coated with a cell wall replicator that does not deform. Scaling up the protoplasm resulted in the expansion and "disintegration" of the cell wall. Flagella was dynamically parented to protoplasm for smooth motion weight gradient. Met with positive reception, but team decided to cut flagella for next rendition0. Rendition also did not include surface proteins.

  1. 3rd Draft - 1st Full Assembly. (07/11/2011 - 07/15/2011)

Rebuilt scene using feedback and lessons from previous renditions.

  1. Visual Overhaul. Used edited version of Yazan Malkosh's electron microscope texture for cell wall platelets
  1. Updated and refined vertex map deformers.
  1. Refined explosion to include organic scaling of bacteria and post-explosion swirling of cell wall platelets and expansion of protoplasm.

 

Mostly positive reception. Team leader wanted more prominent "spikey" deformations at holes, and more of a burst explosion. Consensus  - camera work made bacteria appear to shrink. Also needed to implement surface protein layers.

  1. 4th Draft - 2nd Full Assembly (07/15/2011 - 07/20/2011)

Refined scene

  1. Rebuilt and implemented surface protein vertex map deformers. Protein visuals updated.
  1. Implemented changes to hole deformations. More of a spikey look.
  1. Modified camera work.
  1. Refined Explosion - more of a solid pop and burst.
  1. Updated green light source
  1. Changed protoplasm to a less resource intensive material.

 

Positive reception. Results most satisfactory. Comments that holes were too spikey.

At present, will correct if have time

  1. Experimental Methods (08/08/2011 - present)
    1. Never implemented in-scene. Post-2nd full assembly,  developed a technique for nicer cell wall disintegration around holes. Technique would involve a complete rebuild. If time, plan to implement.

 

Reception and Review: Satisfactory. Potentially scene could use some slight improvements - namely "spikes" and end protoplasm appearance. Otherwise, current result is nicely polished.