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Team:Cornell/Future
From 2011.igem.org
Project Description |
Future Directions |
Business Development |
Outreach/HP |
Safety
Our next goal would be to further reduce the cost of manufacturing this cell-free system. To keep most of our current system's benefits while increasing cost-efficiency, we'd like to describe a novel system involving micro-carriers.
Main System Changes:
- Reaction Chamber: Replace microfluidic chips with large vats
- Enzyme-coated surface: Replace streptavidin-coated microfluidic channels with streptavidin-coated PDMS beads
PDMS beads coated with our avi-tagged enzymes would be placed in large vats. Each vat would contain beads coated with a different enzyme and these vats would be arranged in series according to our substrate's biosynthetic pathway. Mesh filters between vats, will prevent these microcarrier beads traveling across vats. The substrate will sequentially undergo specific reactions in each vat until the final product is obtained.
This system will retain benefits of our existing project:
- Lower purification costs.
- Minimum side reactions.
- Cell-free.
- Modular reactions.
- Continuous, yet modular reactions
Additional benefits:
- Cheaper to scale up manufacturing process.
- Larger reaction volumes.
Potential drawbacks:
- Decrease in solution mixture homogeneity due to larger reaction volumes.
- Less control over reaction conditions.
