Team:Cornell

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<div style="text-align: center;"><big><big><big><a href="https://2011.igem.org/Team:Cornell/Recruiting">Click
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for Recruiting!</a><br><br>
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<font face="Comic Sans MS"><div style="text-align: center;"><strong><big><big><big>Abstract</big></big></big></strong>
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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Cornell’s 2011 iGEM team has designed a new, scalable, and cell-free method to produce complex biomolecules. Current methods for purification from cellular lysate are expensive and time consuming. BioFactory utilizes modified enzymes, capable of being attached to surfaces, in the creation of a modular microfluidic chip for each enzyme. The surface bonding is performed by the well-characterized biotin-avidin mechanism. When combined in series, these chips operate as a linear biochemical pathway for continuous flow reactions. Additionally, we plan to engineer E. coli with the mechanism for light-induced apoptosis to easily lyse cultures producing the necessary enzymes. The resulting lysate is flowed through the microfluidic channels, coating them with the desired enzyme. We believe this chemical synthesis method will reduce unwanted side reactions and lower the costs of producing bio-pharmaceuticals in the future.</p></font>
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Our current proposed project for the summer is the fabrication of a microfluidic device for the cell-free production of bio-pharmaceuticals by binding the necessary enzymes of the pathway to the surface of the channel. The enzymes would be harvested from cellular lysate created by engineering light-induced cell death in bacterial populations which produce the needed enzymes. The hope of the team is to automate and simplify the device construction to allow for a scalable mircofluidic factory.
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|align="center"|[[Team:Cornell | Team Example]]
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!align="center"|[[Team:Cornell|Home]]
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!align="center"|[[Team:Cornell/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Cornell Official Team Profile]
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!align="center"|[[Team:Cornell/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Cornell/Attributions|Attributions]]
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Latest revision as of 01:23, 29 October 2011



Home Table
Click for Recruiting!


Abstract

Cornell’s 2011 iGEM team has designed a new, scalable, and cell-free method to produce complex biomolecules. Current methods for purification from cellular lysate are expensive and time consuming. BioFactory utilizes modified enzymes, capable of being attached to surfaces, in the creation of a modular microfluidic chip for each enzyme. The surface bonding is performed by the well-characterized biotin-avidin mechanism. When combined in series, these chips operate as a linear biochemical pathway for continuous flow reactions. Additionally, we plan to engineer E. coli with the mechanism for light-induced apoptosis to easily lyse cultures producing the necessary enzymes. The resulting lysate is flowed through the microfluidic channels, coating them with the desired enzyme. We believe this chemical synthesis method will reduce unwanted side reactions and lower the costs of producing bio-pharmaceuticals in the future.
Project Data
Team Multimedia


Retrieved from "http://2011.igem.org/Team:Cornell"