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Team:Caltech/Week 8
From 2011.igem.org
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Gibson assemble the PCR'd pNT003 insert and pSB4A5 together if the insert is at a sufficient concentration<br/> | Gibson assemble the PCR'd pNT003 insert and pSB4A5 together if the insert is at a sufficient concentration<br/> | ||
Minimal media transfers<br/> | Minimal media transfers<br/> | ||
| + | Transformation of pUC19 into competent cells for cell dry weight experiment<br/> | ||
===Results=== | ===Results=== | ||
Revision as of 23:37, 1 August 2011
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Project |
July 31Started a LB+amp overnight culture of the B0034 glycerol stock from the 2010 Caltech iGEM Team August 1PCR pSB3K3 and pNT003 insert with the new primers. ResultsThe enrichment culture plates show no visible growth yet. August 2Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3
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