Team:Caltech/Week 4

This Week

Try PCRing from source plate [http://partsregistry.org/Part:pSB4A5 pSB4A5] for Gibson
Gibson assemble pNT001 and pNT002 and negative controls following Joe's protocol.
Transform pNT001 and pNT002 into competent cells
Design experiments to test for degradation of BPA by pNT001 and pNT002 using the HPLC
Get the BioBricks we ordered from the parts registry ([http://partsregistry.org/Part:BBA_K364309 K364309], [http://partsregistry.org/Part:BBA_K364329 K364329], [http://partsregistry.org/Part:BBA_K364327 K364327], [http://partsregistry.org/Part:BBA_K364307 K364307])
Transform these into cells, miniprep and sequence
Continue with enrichment cultures
Put LA River soil DNA into fosmids
Work on Project Description and Safety Proposal
Once we've sequenced ER ([http://partsregitry.org/Part:BBa_K123003 K123003]), start making test plasmids to see if it works.