June 19
Collection of soil and water samples from LA River
June 20
Lab walkthrough
Setting up lab, stock supplies, preparation of LB-amp plates
Test extraction of DNA from BioBrick wells into cloning plates
Initial enrichment cultures of collected samples on minimal media with BPA or 17a-ethynylestradiol
June 21
MoBio kit extraction of DNA from collected samples
Initial enrichment cultures of samples in minimal media with nonylphenol or DDT
Geneious and primer design tutorial
June 22
Miniprep and sequencing of selected BioBricks (K123000, K123001, K123002, K123003)
Preparation of competent cells
June 23
Start cultures of RFP, transformed HER, T7 polymerase
Retest competent cells made June 22 with K123000 and pUC
Transfer 0.5 mL aliquots of BPA and 17a-ethynylestradiol cultures from June 20 to new tubes of minimal media
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