Team:Caltech/Week 2
From 2011.igem.org
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<p>Miniprep and sequencing of selected BioBricks ([http://partsregistry.org/Part:BBa_K123000 K123000], [http://partsregistry.org/Part:BBa_K123001 K123001], [http://partsregistry.org/Part:BBa_K123002 K123002], [http://partsregistry.org/Part:BBa_K123003 K123003]) <br/> | <p>Miniprep and sequencing of selected BioBricks ([http://partsregistry.org/Part:BBa_K123000 K123000], [http://partsregistry.org/Part:BBa_K123001 K123001], [http://partsregistry.org/Part:BBa_K123002 K123002], [http://partsregistry.org/Part:BBa_K123003 K123003]) <br/> | ||
Preparation of competent cells</p> | Preparation of competent cells</p> | ||
- | + | ===Results=== | |
<table border="1"> | <table border="1"> | ||
<tr> | <tr> | ||
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Design primers for test sequence of BisdA (pNT001)<br/> | Design primers for test sequence of BisdA (pNT001)<br/> | ||
Design primers to continue sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003])<br/><br/> | Design primers to continue sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003])<br/><br/> | ||
+ | ===Results=== | ||
We determined that sequences for [http://partsregistry.org/Part:BBa_K123000 K123000] and [http://partsregistry.org/Part:BBa_K123001 K123001] have correct protein coding but different codons than in the sequences shown online </p> | We determined that sequences for [http://partsregistry.org/Part:BBa_K123000 K123000] and [http://partsregistry.org/Part:BBa_K123001 K123001] have correct protein coding but different codons than in the sequences shown online </p> | ||
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Design reverse primer for continued sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003]) <br/> | Design reverse primer for continued sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003]) <br/> | ||
Update Wiki with protocols we have been using<br/><br/> | Update Wiki with protocols we have been using<br/><br/> | ||
+ | ===Results=== | ||
We determined that our competent cells are functional have a cfu/ug DNA of 6.6 x 10^6.</p> | We determined that our competent cells are functional have a cfu/ug DNA of 6.6 x 10^6.</p> | ||
Revision as of 02:45, 28 June 2011
Project |
June 19Collection of soil and water samples from LA River June 20Lab walkthrough June 21MoBio kit extraction of DNA from collected samples June 22Miniprep and sequencing of selected BioBricks (K123000, K123001, K123002, K123003) Results
June 23Transform T7 polymerase (K145001), mCherry (J06702]), Lac Promoter (R0010]), and double terminator (B0014 and B0015) biobricks for test sequences for BisA and BisB degradation genes (K123000 and K123001) ResultsWe determined that sequences for K123000 and K123001 have correct protein coding but different codons than in the sequences shown onlineJune 24Transform Tet repressible promoter R0040 ResultsWe determined that our competent cells are functional have a cfu/ug DNA of 6.6 x 10^6.June 25Take out plates from June 24
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