Team:Caltech/Week 2
From 2011.igem.org
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Transfer 0.5 mL aliquots of BPA and 5mL 17a-ethynylestradiol cultures from June 20 to new tubes of minimal media<br/> | Transfer 0.5 mL aliquots of BPA and 5mL 17a-ethynylestradiol cultures from June 20 to new tubes of minimal media<br/> | ||
Design primers for test sequence of BisdA (pNT001)<br/> | Design primers for test sequence of BisdA (pNT001)<br/> | ||
- | Design primers to continue sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003])<br/> | + | Design primers to continue sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003])<br/><br/> |
We determined that sequences for [http://partsregistry.org/Part:BBa_K123000 K123000] and [http://partsregistry.org/Part:BBa_K123001 K123001] have correct protein coding but different codons than in the sequences shown online </p> | We determined that sequences for [http://partsregistry.org/Part:BBa_K123000 K123000] and [http://partsregistry.org/Part:BBa_K123001 K123001] have correct protein coding but different codons than in the sequences shown online </p> | ||
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Wash dishes from last year <br/> | Wash dishes from last year <br/> | ||
Design reverse primer for continued sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003]) <br/> | Design reverse primer for continued sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003]) <br/> | ||
- | Update Wiki with protocols we have been using</p> | + | Update Wiki with protocols we have been using<br/><br/> |
+ | We determined that our competent cells are functional have a cfu/ug DNA of 6.6 x 10^6.</p> | ||
==June 25== | ==June 25== | ||
- | <p>Take out plates from June 24 </p> | + | <p>Take out plates from June 24</p> |
}} | }} |
Revision as of 02:35, 28 June 2011
Project |
June 19Collection of soil and water samples from LA River June 20Lab walkthrough June 21MoBio kit extraction of DNA from collected samples June 22Miniprep and sequencing of selected BioBricks (K123000, K123001, K123002, K123003)
June 23Transform T7 polymerase (K145001), mCherry (J06702]), Lac Promoter (R0010]), and double terminator (B0014 and B0015) biobricks for test sequences for BisA and BisB degradation genes (K123000 and K123001) June 24Transform Tet repressible promoter R0040 June 25Take out plates from June 24
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