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Team:Caltech/Week 15
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===Results=== | ===Results=== | ||
Estimated concentration of final purified DDT-dechlorinase is .216mg/mL<br/> | Estimated concentration of final purified DDT-dechlorinase is .216mg/mL<br/> | ||
| + | ==September 20== | ||
| + | Miniprepped and sequenced colonies for quikchange.<br/> | ||
| + | 12 reactions, 3 primers for each colony: <br/> | ||
| + | p450qc-1ABC, p450qc-2ABC, p450qc-3ABC, p450qc-4ABC. <br/> | ||
| + | Digest and ligate bisdA, bisdB, & bisdA+bisdB part into correct vector (pSB1C3). <br/> | ||
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Latest revision as of 18:51, 21 September 2011
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Project |
September 18Quikchange of Wt-F87A plasmid and controls, followed by plating ResultsThere are about twenty colonies on the Wt-F87A plates to be sequenced September 19Finish desalting purified DDT-dechlorinase of imidazole ResultsEstimated concentration of final purified DDT-dechlorinase is .216mg/mL September 20Miniprepped and sequenced colonies for quikchange.
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