Team:Bielefeld-Germany/HPLC isocratic

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(Created page with "===HPLC method=== * C18 reverse phase column * Isocratic method: 45% acetonitril * Flow = 0.6 mL/min * UV-detection at 227 n...")
(HPLC method)
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===HPLC method===
===HPLC method===
* C18 reverse phase column
* C18 reverse phase column
-
* Isocratic method: 45% [[Team:Bielefeld-Germany/Protocols#Used_chemicals | acetonitril]]
+
* Isocratic method: 45% [[Team:Bielefeld-Germany/Protocols#Used_chemicals | acetonitrile]]
* Flow = 0.6 mL/min
* Flow = 0.6 mL/min
* UV-detection at 227 nm
* UV-detection at 227 nm

Revision as of 09:22, 23 August 2011

HPLC method

  • C18 reverse phase column
  • Isocratic method: 45% acetonitrile
  • Flow = 0.6 mL/min
  • UV-detection at 227 nm
  • Column:
    • Eurospher II 100-5 C18p
    • Dimensions: 150 x 4.6 mm with precolumn
    • Particle size: 5 µm
    • Pore size: 100 Å
    • Material: silica gel