Team:Bielefeld-Germany/HPLC isocratic

From 2011.igem.org

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===HPLC method===
===HPLC method===
* C18 reverse phase column
* C18 reverse phase column
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* Isocratic method: 45 % [[Team:Bielefeld-Germany/Protocols#Used_chemicals | acetonitrile]]
+
* Isocratic method: 45 % [[Team:Bielefeld-Germany/Protocols#Used_chemicals | Acetonitrile]]
* Flow = 0.6 mL min<sup>-1</sup>
* Flow = 0.6 mL min<sup>-1</sup>
* UV-detection at 227 nm
* UV-detection at 227 nm
 +
* Internal standard: 100 mg <sup>-1</sup> [[Team:Bielefeld-Germany/Protocols#Used_chemicals | Bisphenol F]]
* Column:
* Column:
** Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer]
** Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer]
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[[File:Bielefeld2011 BPA + Abbauprodukt-2.JPG|900px|left|thumb|Chromatogram obtained using the above described materials and methods. Three distinctive Peak are of interest: BPA at 7,3 min retention time, BPF at 5,8 min retention time and the degradation product of BPA at 4,6 min retention time]]
[[File:Bielefeld2011 BPA + Abbauprodukt-2.JPG|900px|left|thumb|Chromatogram obtained using the above described materials and methods. Three distinctive Peak are of interest: BPA at 7,3 min retention time, BPF at 5,8 min retention time and the degradation product of BPA at 4,6 min retention time]]
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[[File:Bielefeld2011 BPA + Abbauprodukt-3.JPG|900px]]
 
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[[File:Bielefeld2011 BPA + Abbauprodukt.JPG|900px]]
 

Revision as of 13:42, 31 August 2011

HPLC method

  • C18 reverse phase column
  • Isocratic method: 45 % Acetonitrile
  • Flow = 0.6 mL min-1
  • UV-detection at 227 nm
  • Internal standard: 100 mg -1 Bisphenol F
  • Column:
    • Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer]
    • Dimensions: 150 x 4.6 mm with precolumn
    • Particle size: 5 µm
    • Pore size: 100 Å
    • Material: silica gel
  • Software:
    • Clarity (Version 3.0.5.505) by [http://www.dataapex.com/ Data Apex]
  • Autosampler:
    • Midas by [http://www.spark.nl/ Spark Holland]
    • Tray cooling: 10 °C
  • Pump:
    • L-6200A Intelligent Pump by [http://www.hitachi.com/ Hitachi]
  • UV-Detector:
    • Series 1050 by [http://www.hp.com/ Hewlett Packard]
Chromatogram obtained using the above described materials and methods. Three distinctive Peak are of interest: BPA at 7,3 min retention time, BPF at 5,8 min retention time and the degradation product of BPA at 4,6 min retention time
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