Team:Bielefeld-Germany/HPLC isocratic

From 2011.igem.org

(Difference between revisions)
(HPLC method)
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* UV-Detector:
* UV-Detector:
** Series 1050 by [http://www.hp.com/ Hewlett Packard]
** Series 1050 by [http://www.hp.com/ Hewlett Packard]
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Revision as of 14:29, 29 August 2011

HPLC method

  • C18 reverse phase column
  • Isocratic method: 45 % acetonitrile
  • Flow = 0.6 mL min-1
  • UV-detection at 227 nm
  • Column:
    • Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer]
    • Dimensions: 150 x 4.6 mm with precolumn
    • Particle size: 5 µm
    • Pore size: 100 Å
    • Material: silica gel
  • Software:
    • Clarity (Version 3.0.5.505) by [http://www.dataapex.com/ Data Apex]
  • Autosampler:
    • Midas by [http://www.spark.nl/ Spark Holland]
    • Tray cooling: 10 °C
  • Pump:
    • L-6200A Intelligent Pump by [http://www.hitachi.com/ Hitachi]
  • UV-Detector:
    • Series 1050 by [http://www.hp.com/ Hewlett Packard]

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