"
Team:Bielefeld-Germany/HPLC isocratic
From 2011.igem.org
(Difference between revisions)
JSchwarzhans (Talk | contribs) |
JSchwarzhans (Talk | contribs) (→HPLC method) |
||
| Line 2: | Line 2: | ||
* C18 reverse phase column | * C18 reverse phase column | ||
* Isocratic method: 45 % [[Team:Bielefeld-Germany/Protocols#Used_chemicals | acetonitrile]] | * Isocratic method: 45 % [[Team:Bielefeld-Germany/Protocols#Used_chemicals | acetonitrile]] | ||
| - | * Flow = 0.6 mL | + | * Flow = 0.6 mL min<sup>-1</sup> |
* UV-detection at 227 nm | * UV-detection at 227 nm | ||
* Column: | * Column: | ||
| Line 10: | Line 10: | ||
** Pore size: 100 Å | ** Pore size: 100 Å | ||
** Material: silica gel | ** Material: silica gel | ||
| + | * Software: | ||
| + | ** Clarity (Version 3.0.5.505) by [http://www.dataapex.com/ Data Apex] | ||
| + | * Autosampler: | ||
| + | ** Midas by [http://www.spark.nl/ Spark Holland] | ||
| + | ** Tray cooling: 10 °C | ||
| + | * Pump: | ||
| + | ** L-6200A Intelligent Pump by [http://www.hitachi.com/ Hitachi] | ||
| + | * UV-Detector: | ||
| + | ** Series 1050 by [http://www.hp.com/ Hewlett Packard] | ||
Revision as of 13:46, 29 August 2011
HPLC method
- C18 reverse phase column
- Isocratic method: 45 % acetonitrile
- Flow = 0.6 mL min-1
- UV-detection at 227 nm
- Column:
- Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer]
- Dimensions: 150 x 4.6 mm with precolumn
- Particle size: 5 µm
- Pore size: 100 Å
- Material: silica gel
- Software:
- Clarity (Version 3.0.5.505) by [http://www.dataapex.com/ Data Apex]
- Autosampler:
- Midas by [http://www.spark.nl/ Spark Holland]
- Tray cooling: 10 °C
- Pump:
- L-6200A Intelligent Pump by [http://www.hitachi.com/ Hitachi]
- UV-Detector:
- Series 1050 by [http://www.hp.com/ Hewlett Packard]
