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- Team:Potsdam Bioware/Labjournal/August part 2 ...er contains a complete myc tag for insertion of myc into the phage display vector * this may lead to the low yield of geneIII target DNA after PCR using entire vector pak bla KDIR as template134 KB (19,391 words) - 13:03, 21 September 2011
- Team:Potsdam Bioware/Labjournal/September part 1 ...ackground-color: rgb(254, 122, 122); font-weight: bold;">Digest of pUP089 (vector) </h3> <b>Aim:</b> generate vector for ligation and transformation to generate a libary151 KB (21,501 words) - 17:06, 21 September 2011
- Team:TU Munich/lab/notebook/part3 ...st:</p><p>pSB3C5: S and P cut by Alex on 23.08.11, CAM resistance, 2,7 kbp vector backbone</p><p>PCR product of BBa_K322127 with primers BBa_K568000, derived ...he following bands were expected: 3.1 kbp for lacZ with rbs and 2 kbp for vector pSB1A2. 3.4 kbp for T7prom in pSB1AK8. The bands are excised from the gel a121 KB (20,136 words) - 01:54, 22 September 2011
- Team:Edinburgh/Lab Notebook ...e (so the final thing becomes E-X-B-Plac-lacZα-S-P). This gives us a vector that we like. For the principles behind the use of lacZα see [http:// ...y DNA sequence can easily have the sites needed for incorporation into the vector added (by PCR) at both ends, whereas in the standard RFC10 system the two m69 KB (11,148 words) - 15:33, 3 October 2011
- Team:Potsdam Bioware/Labjournal/September part 2 ...yle="background-color: rgb(254, 122, 122); font-weight: bold;">miniprep of pSB1C3+mdnABCDE with and without T7 promotor</h3> <b>Aim:</b> generate BioBrick of mdnABCDE, amplify mdnABCDE for ligation in pSB1C3<br>58 KB (8,180 words) - 00:26, 22 September 2011
- Team:TU Munich/lab/notebook/part4 ...ee samples of pSB6A1 with I746907, different I:V-ratios; pSB6A1 E,P cut as vector background) was purified today using glycogen/ethanol precipitation accordi Plate 4 (vector background) spread with concentrated bacteria solution showed 85 colonies.78 KB (13,436 words) - 22:42, 28 October 2011
- Team:Potsdam Bioware/Labjournal/August part 1 <b>Further tasks:</b><br> ligation into vector * 6 µl (ca 70 ng) digested vector pARW089 (NarI, AatII)117 KB (17,234 words) - 01:03, 22 September 2011
- Team:Uppsala-Sweden/Notebook pSB1C3-J04450 (CmR backbone) – Lei & Sibel <br> pSB3T5-J04450 (low copy vector tet, ori P15A) – From Erik G <br>55 KB (8,809 words) - 15:16, 25 October 2011
- Team:KIT-Kyoto/Notebook/LabNote/GFPMLF9 ...ickgelextractionkit.aspx|QIAquick Gel Extraction Kit] and extracted DNA of pSB1C3 from the gel. ...ickgelextractionkit.aspx|QIAquick Gel Extraction Kit] and extracted DNA of pSB1C3 from the gel.37 KB (6,399 words) - 03:45, 6 October 2011
- Team:NTNU Trondheim/Journal *6:1 molar ratio of insert to vector (~10ng vector) *Add (8.5 - vector and insert volume)μl ddH2O64 KB (10,273 words) - 15:51, 21 September 2011
- Team:Nevada/Notebook/Weeks912 ...ata confirms the TRC promoter was successfully cloned into the TOPO2.1 PCR vector! ...I and checked on 0.7% gel. The results for this gel were good. ThiE/Topo Vector was digest with EcoRI and PstI and checked on a 0.7% gel. The gel was not30 KB (5,035 words) - 03:20, 29 September 2011
- Team:Potsdam Bioware/Labjournal/June * Sequence of vector pARW071, concentration 857.3 ng/ µl * Sequence of vector pARW089, concentration 626.7 ng/ µl70 KB (9,678 words) - 01:31, 22 September 2011
- Team:UNICAMP-EMSE Brazil/Notebook/17 September 2011 :# Quantification of linearized pSB1C3 vector purified from the gel (volume of ladder applied 6 uL; sample: 5 uL DNA +1 u ! scope="col" style="background:#efefef;" | Total size (gene+vector)14 KB (2,168 words) - 14:09, 19 September 2011
- Team:UT-Tokyo/LabNote | || || || pSB1C3 || class="number" | 831 | pSB1C3 || || Linearized plasmid backbone || pSB1C3 || class="number" | 2070186 KB (30,010 words) - 20:25, 5 October 2011
- Team:Nevada/Notebook/Weeks1316 ...in the Topo vector (pUC57) were grown, and minipreped. The BTE samples and pSB1C3 were digested with Xba I and Pst I and ligated. Ligation was transformed u BTE with sigma 70 promoter and pSB1C3 were digested with EcoRI and PstI and ligated, transformed, and cultured.<b25 KB (4,235 words) - 03:58, 29 September 2011
- Team:Lethbridge/Notebook/Lab Work/Group1 <i>Transformation of K325909 in pSB1C3 and K325229 in pSB1C3 from 2011 Kit Plate</i> * 10µL of Milli-Q water and extraction of K325909 in pSB1C3 from the 2011 kit plate (17m) were mixed together.22 KB (3,366 words) - 03:32, 29 September 2011
- Team:Potsdam Bioware/Labjournal/October * Ligation with pSB1C3 * Ligation with pSB1C389 KB (13,149 words) - 18:03, 28 October 2011
- Team:UNIPV-Pavia/Freezer <td class="row"><b>Vector</b></td> <td class="row">Low copy BioBrick standard vector - chloramphenicol antibiotic resistance</td>31 KB (4,892 words) - 13:30, 9 November 2011
- Team:UPO-Sevilla/Project/Notebook/MiniTn7 ... replicative in Enterobacteriaceae to maintain the mini-Tn7. I located the vector in the lab strain collection, set up an inoculum, purified the plasmid DNA, ...ab in Colorado State University. We will use this as a template to amplify vector sequences adding SfiI restriction sites. I had problems to get amplificatio35 KB (5,556 words) - 10:07, 28 October 2011
- Team:UNIPV-Pavia/Calendar/August/week4 <td class="row"><b>Vector</b></td> <td class="row"><b>Vector volume (μl)</b></td>18 KB (3,015 words) - 10:12, 18 September 2011
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