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- 04:37, 29 September 2011 (diff | hist) Team:Grinnell/Project (→The Experiments)
- 04:30, 29 September 2011 (diff | hist) Team:Grinnell/Project (→Biofilms)
- 04:23, 29 September 2011 (diff | hist) Team:Grinnell/Project (→Data & Results)
- 04:19, 29 September 2011 (diff | hist) Team:Grinnell/Project (→Data & Results)
- 04:12, 29 September 2011 (diff | hist) Team:Grinnell/Notebook/Protocols (top)
- 04:07, 29 September 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 04:03, 29 September 2011 (diff | hist) Team:Grinnell/Project/RsaA (→RsaA) (top)
- 04:02, 29 September 2011 (diff | hist) Team:Grinnell/Project/RsaA
- 03:59, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home (top)
- 03:57, 29 September 2011 (diff | hist) Team:Grinnell
- 03:57, 29 September 2011 (diff | hist) Team:Grinnell
- 03:55, 29 September 2011 (diff | hist) Team:Grinnell
- 03:53, 29 September 2011 (diff | hist) Team:Grinnell
- 03:51, 29 September 2011 (diff | hist) Team:Grinnell
- 03:49, 29 September 2011 (diff | hist) Team:Grinnell
- 03:39, 29 September 2011 (diff | hist) N File:CaulobacterButton.jpg (top)
- 03:39, 29 September 2011 (diff | hist) N File:NotebookButton.jpg (top)
- 03:37, 29 September 2011 (diff | hist) N File:BiofilmButton.jpg (top)
- 03:23, 29 September 2011 (diff | hist) Team:Grinnell/Project (→Background)
- 03:01, 29 September 2011 (diff | hist) N File:Esp.png (top)
- 03:01, 29 September 2011 (diff | hist) N File:DspB.jpg (top)
- 02:48, 29 September 2011 (diff | hist) Team:Grinnell/Project
- 02:41, 29 September 2011 (diff | hist) Team:Grinnell/Project (→Project)
- 02:40, 29 September 2011 (diff | hist) Team:Grinnell
- 02:38, 29 September 2011 (diff | hist) Team:Grinnell/Project (→Project Details)
- 02:16, 29 September 2011 (diff | hist) Team:Grinnell/Project (→Results)
- 02:14, 29 September 2011 (diff | hist) Team:Grinnell/Project (→Results)
- 02:13, 29 September 2011 (diff | hist) Team:Grinnell/Attributions
- 02:11, 29 September 2011 (diff | hist) Team:Grinnell/Attributions
- 02:09, 29 September 2011 (diff | hist) Team:Grinnell/Attributions
- 01:54, 29 September 2011 (diff | hist) File:PGel2 Labeled.png (uploaded a new version of "File:PGel2 Labeled.png") (top)
- 01:53, 29 September 2011 (diff | hist) File:PGel1 Labeled.png (uploaded a new version of "File:PGel1 Labeled.png") (top)
- 01:53, 29 September 2011 (diff | hist) File:GroupPhoto.jpg (uploaded a new version of "File:GroupPhoto.jpg") (top)
- 01:49, 29 September 2011 (diff | hist) File:GroupPhoto.jpg (uploaded a new version of "File:GroupPhoto.jpg")
- 01:38, 29 September 2011 (diff | hist) Team:Grinnell/Team (top)
- 01:37, 29 September 2011 (diff | hist) Team:Grinnell/Team
- 01:27, 29 September 2011 (diff | hist) Team:Grinnell/Project
- 01:26, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:26, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:25, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:25, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:21, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:18, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:17, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:17, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:16, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:16, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:15, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:10, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:05, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 00:56, 29 September 2011 (diff | hist) Team:Grinnell/Project (→The Experiments)
- 00:08, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 00:08, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 00:07, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 00:07, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 00:06, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 00:04, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 00:04, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 00:03, 29 September 2011 (diff | hist) Team:Grinnell/Team
- 00:03, 29 September 2011 (diff | hist) Team:Grinnell/Team
- 00:01, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 00:00, 29 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 23:57, 28 September 2011 (diff | hist) Team:Grinnell
- 23:51, 28 September 2011 (diff | hist) Team:Grinnell/Project/RsaA
- 23:49, 28 September 2011 (diff | hist) Team:Grinnell/Attributions
- 23:49, 28 September 2011 (diff | hist) Team:Grinnell/Attributions
- 23:42, 28 September 2011 (diff | hist) N File:BiofilmBuster.jpg (Art courtesy of MB.) (top)
- 23:27, 28 September 2011 (diff | hist) Team:Grinnell/Project (→Project Details)
- 23:26, 28 September 2011 (diff | hist) Team:Grinnell/Project (→Project Details)
- 23:24, 28 September 2011 (diff | hist) Team:Grinnell/Project (→Project Details)
- 23:24, 28 September 2011 (diff | hist) Team:Grinnell/Project (→Project Details)
- 23:05, 28 September 2011 (diff | hist) Team:Grinnell/Project
- 23:05, 28 September 2011 (diff | hist) Team:Grinnell/Project
- 23:05, 28 September 2011 (diff | hist) Team:Grinnell/Project
- 22:38, 28 September 2011 (diff | hist) Team:Grinnell/Project/DspB (top)
- 22:38, 28 September 2011 (diff | hist) Team:Grinnell/Attributions
- 20:37, 28 September 2011 (diff | hist) Team:Grinnell/Attributions
- 19:55, 28 September 2011 (diff | hist) Team:Grinnell/Recipes (top)
- 19:09, 28 September 2011 (diff | hist) Team:Grinnell/Attributions
- 14:05, 28 September 2011 (diff | hist) Team:Grinnell/Attributions
- 14:03, 28 September 2011 (diff | hist) Team:Grinnell/Attributions
- 14:02, 28 September 2011 (diff | hist) Team:Grinnell/Attributions
- 05:14, 28 September 2011 (diff | hist) Team:Grinnell/Project (→Overall project)
- 04:22, 28 September 2011 (diff | hist) N File:PGel2 Labeled.png (Created using ImageJ and Microsoft Office's Powerpoint)
- 04:21, 28 September 2011 (diff | hist) N File:PGel1 Labeled.png (Created using ImageJ and Microsoft Office's Powerpoint)
- 04:14, 27 September 2011 (diff | hist) Team:Grinnell CN/Notebook/Gels/Promoters (top)
- 04:14, 27 September 2011 (diff | hist) Team:Grinnell CN/Notebook/Gels/Esp (top)
- 04:14, 27 September 2011 (diff | hist) Team:Grinnell CN/Notebook/Gels/DspB (top)
- 04:14, 27 September 2011 (diff | hist) Team:Grinnell CN/Notebook/Gels (top)
- 04:14, 27 September 2011 (diff | hist) Team:Grinnell CN/Recipes (top)
- 04:14, 27 September 2011 (diff | hist) Team:Grinnell CN/Notebook/Protocols (top)
- 04:14, 27 September 2011 (diff | hist) Team:Grinnell CN/Safety (top)
- 04:14, 27 September 2011 (diff | hist) Team:Grinnell CN/Notebook (top)
- 04:13, 27 September 2011 (diff | hist) Team:Grinnell CN/Attributions/Sponsors (top)
- 04:13, 27 September 2011 (diff | hist) Team:Grinnell CN/Attributions (top)
- 04:13, 27 September 2011 (diff | hist) Team:Grinnell CN/Project/RsaA (top)
- 04:13, 27 September 2011 (diff | hist) Team:Grinnell CN/Project/Esp (top)
- 04:13, 27 September 2011 (diff | hist) Team:Grinnell CN/Project/DspB (top)
- 04:12, 27 September 2011 (diff | hist) Team:Grinnell CN/Parts (top)
- 04:12, 27 September 2011 (diff | hist) Team:Grinnell CN/Applications (top)
- 04:12, 27 September 2011 (diff | hist) Team:Grinnell CN/Project (top)
- 04:12, 27 September 2011 (diff | hist) Team:Grinnell CN/Gallery (top)
- 04:11, 27 September 2011 (diff | hist) Team:Grinnell CN/Team (top)
- 04:11, 27 September 2011 (diff | hist) Team:Grinnell CN/Template/Home (top)
- 04:11, 27 September 2011 (diff | hist) Team:Grinnell CN (top)
- 04:07, 27 September 2011 (diff | hist) Team:Grinnell CN/Template/Home
- 04:07, 27 September 2011 (diff | hist) Team:Grinnell CN/Template/Home
- 04:06, 27 September 2011 (diff | hist) N Team:Grinnell CN/Template/Home (Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=windows-31j"> <title>Grinnell Menubar</title> <style type="text/css"> /*Roughly inspired by JHU iGEM...")
- 04:03, 27 September 2011 (diff | hist) Team:Grinnell CN
- 04:02, 27 September 2011 (diff | hist) N Team:Grinnell CN/Gallery (Created page with "{{:Team:Grinnell/Template/Home}} =Team Gallery= <html> <embed type="application/x-shockwave-flash" src="https://picasaweb.google.com/s/c/bin/slideshow.swf" width="800" height="60...")
- 04:01, 27 September 2011 (diff | hist) N Team:Grinnell CN/Project (Created page with "{{:Team:Grinnell/Template/Home}} =Project= File:Project.jpg == '''Overall project''' == Your abstract == Project Details== [[Team:Grinnell/Project/DspB|''DspB...")
- 04:01, 27 September 2011 (diff | hist) N Team:Grinnell CN/Applications (Created page with "{{:Team:Grinnell/Template/Home}} =Applications= Biofilms are a type of bacterial community caused by cells adhering to a surface, multiplying and embedding themselves in a matrix...")
- 04:00, 27 September 2011 (diff | hist) N Team:Grinnell CN/Parts (Created page with "{{:Team:Grinnell/Template/Home}} =Parts= ===Parts=== <groupparts>iGEM011 Grinnell</groupparts>")
- 04:00, 27 September 2011 (diff | hist) N Team:Grinnell CN/Project/DspB (Created page with "{{:Team:Grinnell/Template/Home}} =DspB= DspB has been isolated and purified and has been shown to increase dissemination of wild type cells from biofilms and restore dissemina...")
- 03:59, 27 September 2011 (diff | hist) N Team:Grinnell CN/Project/Esp (Created page with "{{:Team:Grinnell/Template/Home}} =Esp= This is a page where you get to learn about the inner workings of the suspected serine protease known as Esp.")
- 03:59, 27 September 2011 (diff | hist) N Team:Grinnell CN/Project/RsaA (Created page with "{{:Team:Grinnell/Template/Home}} =RsaA= <html> <p>RsaA is the surface-layer (S-layer) protein produced by the gram-negative bacteria <i>Caulobacter crescentus</i>. It covers th...")
- 03:58, 27 September 2011 (diff | hist) N Team:Grinnell CN/Notebook (Created page with "{{:Team:Grinnell/Template/Home}} <html><head><style type="text/css"> ul.figure{ list-style:none; float:right; width:170px; } </style></head></html> =Notebook= <html><ul class="f...")
- 03:58, 27 September 2011 (diff | hist) N Team:Grinnell CN/Safety (Created page with "{{:Team:Grinnell/Template/Home}} =Safety= <html> <head><style type="text/css"> ol li b{ font-size:120%; } ol li ul li{ font-size:110%; } ol li ul p{ fon...")
- 03:57, 27 September 2011 (diff | hist) N Team:Grinnell CN/Notebook/Protocols (Created page with "{{:Team:Grinnell/Template/Home}} =Protocols= <html> <head><style type="text/css"> td{text-align:center;} </style></head><body> <h2><a name="Competent"></a>Competent Cells</h2> <...")
- 03:57, 27 September 2011 (diff | hist) N Team:Grinnell CN/Recipes (Created page with "{{:Team:Grinnell/Template/Home}} <html> <head> <style type="text/css"> th{ width:100px; } td{ text-align:center; } </style> </head <body> <h1>Recipes</h1> <h2><a name="LB">...")
- 03:56, 27 September 2011 (diff | hist) N Team:Grinnell CN/Notebook/Gels (Created page with "{{:Team:Grinnell/Template/Home}} <html> <body> <h1>Gel Pics</h1> <div style="float:right; position:relative; left:-125px; top:-5px; z-index:-1"> <img alt="Our first gel" src='htt...")
- 03:56, 27 September 2011 (diff | hist) N Team:Grinnell CN/Notebook/Gels/DspB (Created page with "{{:Team:Grinnell/Template/Home}} {{:Team:Grinnell/Template/Gallery}} =Gels for DspB= ==2011.07.03-2011.07.09== <html> <table class="gallery" frame='void' rules='none'> <tr>...")
- 03:55, 27 September 2011 (diff | hist) N Team:Grinnell CN/Notebook/Gels/Esp (Created page with "{{:Team:Grinnell/Template/Home}} {{:Team:Grinnell/Template/Gallery}} =Gels for Esp= ==2011.06.05-2011.06.11== <html> <table class="gallery" frame='void' rules='none'> <tr> ...")
- 03:55, 27 September 2011 (diff | hist) N Team:Grinnell CN/Notebook/Gels/Promoters (Created page with "{{:Team:Grinnell/Template/Home}} {{:Team:Grinnell/Template/Gallery}} =Gels for Promoters= ==2011.06.12-2011.06.18== <html> <table class="gallery" frame='void' rules='none'> ...")
- 03:54, 27 September 2011 (diff | hist) N Team:Grinnell CN/Attributions (Created page with "{{:Team:Grinnell/Template/Home}} =Attributions= ==References== ===<html><a name="dspB"></a></html>DspB References=== 1) '''Simões, M., L. Simões, and M.J. Vieira...")
- 03:53, 27 September 2011 (diff | hist) N Team:Grinnell CN/Attributions/Sponsors (Created page with "{{:Team:Grinnell/Template/Home}} =Thanks to our Sponsors= We thank <html><a href='http://www.idtdna.com/Home/Home.aspx'>Integrated DNA Technologies (IDT)</a></html> for generous...")
- 03:52, 27 September 2011 (diff | hist) N Team:Grinnell CN/Team (Created page with "{{:Team:Grinnell/Template/Home}} <html> <head> <meta http-equiv="Content-Type" content="text/html; charset=windows-31j"> <title>Team Grinnell</title> <style type="text/css"> i...")
- 03:50, 27 September 2011 (diff | hist) N Team:Grinnell CN (Created page with "Home page")
- 14:01, 26 September 2011 (diff | hist) Team:Grinnell/Attributions/Sponsors (top)
- 04:36, 26 September 2011 (diff | hist) Team:Grinnell/Team
- 04:35, 26 September 2011 (diff | hist) Team:Grinnell/Team
- 04:31, 26 September 2011 (diff | hist) File:LB Profile300.jpg (uploaded a new version of "File:LB Profile300.jpg": Correct proportions.) (top)
- 04:27, 26 September 2011 (diff | hist) N File:QG Profile300.jpg (Qimeng. Nothing else need be said.) (top)
- 04:26, 26 September 2011 (diff | hist) N File:NK Profile300.jpg (Interesting...) (top)
- 04:25, 26 September 2011 (diff | hist) N File:LB Profile300.jpg (Safety first.)
- 04:25, 26 September 2011 (diff | hist) N File:AA Profile300.jpg (Huh?) (top)
- 03:15, 26 September 2011 (diff | hist) File:GroupPhoto.jpg
- 03:14, 26 September 2011 (diff | hist) N File:GroupPhoto.jpg (Right to left: Alex Aaring, Dr. Lisa Bowers, Nora Kostow, Qimeng Gao)
- 22:33, 25 September 2011 (diff | hist) Team:Grinnell/Template/Home
- 22:18, 25 September 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 23:56, 2 September 2011 (diff | hist) Team:Grinnell/Safety (→Safety) (top)
- 23:55, 2 September 2011 (diff | hist) Team:Grinnell/Safety
- 23:54, 2 September 2011 (diff | hist) Team:Grinnell/Safety (→Safety)
- 03:51, 2 September 2011 (diff | hist) Team:Grinnell/Safety
- 21:33, 1 August 2011 (diff | hist) File:20110725 BBa+odspB+rsaA c10-17.jpg
- 21:32, 1 August 2011 (diff | hist) Team:Grinnell/Notebook/Gels/DspB (→2011.07.24-2011.07.31)
- 21:32, 1 August 2011 (diff | hist) Team:Grinnell/Notebook/Gels/Esp (→2011.07.24-2011.08.01) (top)
- 21:30, 1 August 2011 (diff | hist) Team:Grinnell/Notebook/Gels/Esp (→2011.07.17-2011.07.23)
- 21:28, 1 August 2011 (diff | hist) N File:20110801 BBa+oesp+rsaA+pMR10.jpg (Lane 1: ladder; lanes 2-10: transformants that should contain BBa_K081005, optimized <i>esp</i>, and <i>rsaA</i>. Lanes 2 and 8 show success.) (top)
- 21:26, 1 August 2011 (diff | hist) N File:20110729 BBa+oesp+rsaA+pMR10 C3.jpg (Lane 1: ladder; lanes 2,3: PCR of transformants that should carry BBa_K081005, optimized <i>esp</i>, and <i>rsaA</i> in pMR10; lanes 4-10: PCR of transformants that should carry BBa_K081005, optimized <i>esp</i>, and <i>rsaA</i> in pSB1C3. No success.) (top)
- 21:24, 1 August 2011 (diff | hist) N File:20110728 BD1 Caulobacter.jpg (Lane 1: ladder; lanes 2-5: PCR of <i>Caulobacter</i> colonies believed to carry BBa_K081005, WT <i>esp</i>, and <i>rsaA</i>. All passed.) (top)
- 21:21, 1 August 2011 (diff | hist) N File:20110727 BBa+oesp+rsaA.jpg (Lane 1: ladder; lanes 2-8: digests of miniprep of transformants that should carry BBa_K081005, optimized <i>esp</i>, and <i>rsaA</i>. No success.) (top)
- 21:18, 1 August 2011 (diff | hist) N File:20110725 Pxyl+oesp+rsaA+pMR10.jpg (Lane 1: ladder; lanes 2-8: digests of transformants that should contain P<sub>xyl</sub>, optimized <i>esp<i>, and <i>rsaA</i>.) (top)
- 21:10, 1 August 2011 (diff | hist) Team:Grinnell/Notebook/Gels/DspB
- 21:09, 1 August 2011 (diff | hist) N File:20110725 BBa+odspB+rsaA c10-17.jpg (Lane 1: ladder; lanes 2-9: colony PCR of transformants that should contain BBa_K081005, optimized dspB, and rsaA.)
- 21:03, 1 August 2011 (diff | hist) Team:Grinnell/Notebook/Gels/DspB (→2011.07.17-2011.07.23)
- 13:50, 29 July 2011 (diff | hist) N Team:Grinnell/Template/Base (Created page with "<!--For use as a footer in conjunction with Template/Home-->") (top)
- 16:42, 28 July 2011 (diff | hist) Team:Grinnell/Template/Test (top)
- 16:29, 28 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 16:17, 28 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 15:54, 28 July 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 15:20, 28 July 2011 (diff | hist) Team:Grinnell/Safety
- 15:18, 28 July 2011 (diff | hist) Team:Grinnell/Team
- 03:33, 28 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 22:01, 27 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 19:45, 27 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 15:56, 27 July 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 14:02, 27 July 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 21:20, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:20, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:20, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:20, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:20, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:19, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:19, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:19, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:19, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:19, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:18, 26 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 18:17, 26 July 2011 (diff | hist) Team:Grinnell/Project/RsaA
- 18:12, 26 July 2011 (diff | hist) Team:Grinnell/Attributions (→RsaA References)
- 18:11, 26 July 2011 (diff | hist) Team:Grinnell/Attributions (→RsaA References)
- 18:11, 26 July 2011 (diff | hist) Team:Grinnell/Attributions (→RsaA References)
- 18:09, 26 July 2011 (diff | hist) Team:Grinnell/Project/Esp (→Esp)
- 18:08, 26 July 2011 (diff | hist) Team:Grinnell/Project/Esp
- 18:07, 26 July 2011 (diff | hist) N Team:Grinnell/Project/Esp (Created page with "{{Team:Grinnell/Template/Home}} =Esp=")
- 18:07, 26 July 2011 (diff | hist) Team:Grinnell/Team (→What we did)
- 18:45, 25 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 18:44, 25 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 18:44, 25 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 18:43, 25 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 18:43, 25 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 18:43, 25 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 18:43, 25 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 18:42, 25 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 18:41, 25 July 2011 (diff | hist) N File:Grinnell wiki logo 975px.jpg (top)
- 18:40, 25 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 17:03, 25 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/Esp (→2011.07.17-2011.07.23)
- 16:58, 25 July 2011 (diff | hist) N File:20110723 Pxyl+oesp+rsaA+pMR10.jpg (Lane 1: ladder; lanes 2-7: PCR of transformants that should carry P<sub>xyl</sub>, optimized <i>esp</i>, and <i>rsaA</i> in pMR10.) (top)
- 16:57, 25 July 2011 (diff | hist) N File:20110723 PrsaA+oesp+rsaA+pMR10.jpg (Lane 1: ladder; lanes 2-7: PCR of transformants that should carry P<sub>rsaA</sub>, optimized <i>esp</i>, and <i>rsaA</i> in pMR10. Lane 4 was successful.) (top)
- 16:55, 25 July 2011 (diff | hist) N File:20110723 BBa+oesp.jpg (Gel of digests of miniprepped overnight liquid culture of cells that should carry BBa_K081005 and optimized <i>esp</i>. Lane 1: ladder; lanes 2-10: digests.) (top)
- 16:52, 25 July 2011 (diff | hist) N File:20110722 Bba+oesp.jpg (Lane 1: ladder; lanes 2-7: transformants that should carry BBa_K081005 and optimized <i>esp</i>.) (top)
- 16:51, 25 July 2011 (diff | hist) N File:20110720 Pxyl+oesp+rsaA oesp+rsaAC3.jpg (Lane 1: ladder; lanes 2-7: PCR of transformants that should carry P<sub>xyl</sub>, optimized <i>esp</i>, and <i>rsaA</i>; lanes 8-10: transformants that should carry optimized <i>esp</i> with <i>rsaA</i> in pSB1C3.) (top)
- 16:48, 25 July 2011 (diff | hist) N File:20110720 PrsaA+oesp+rsaA oesp+rsaAC3.jpg (PCR of transformants that should carry P<sub>rsaA</sub>, optimized <i>esp</i>, and <i>rsaA</i> or optimized <i>esp</i> with <i>rsaA</i> in pSB1C3. Lane 1: ladder; lanes 2-7:combo with P<sub>rsaA</sub>; lanes 8-10: combo without promoter. Lanes 3, 8, and 1) (top)
- 16:39, 25 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 16:36, 25 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/DspB (→2011.07.17-2011.07.23)
- 16:29, 25 July 2011 (diff | hist) N File:20110722 Pxyl+odspB+rsaA+pMR10.jpg (PCR of transformants that should carry P<sub>xyl</sub>, optimized <i>dspB</i>, and <i>rsaA</i> in pMR10. Lane 1: ladder; lanes 2-8: PCR products.)
- 16:27, 25 July 2011 (diff | hist) N File:20110722 BBa+odspB+rsaA+pMR10 c1,5-9.jpg (PCR of transformants that should carry BBa_K081005, optimized <i>dspB</i>, and <i>rsaA</i> in pMR10. Lane 1: ladder; lanes 2-7: PCR products.) (top)
- 16:25, 25 July 2011 (diff | hist) N File:20110720 Pxyl+odspB+rsaA re-do c7-14 WTdspB c5.jpg (Gel of PCR of transformants that should carry P<sub>xyl</sub>, optimized <i>dspB</i>, and <i>rsaA</i>. Lane 1: ladder; lanes 2-9: transformant PCR products; lane 10: digested miniprep of what should have been WT <i>dspB</i>, but showed up as the wrong fra)
- 16:16, 25 July 2011 (diff | hist) N File:20110719 Pxyl+odspB+rsaA re-do c1,3,4,5,6 std.jpg (Gel of PCR of transformants that should carry P<sub>xyl</sub>, optimized <i>dspB</i>, and <i>rsaA</i>. Lane 1: ladder; lanes 2-6: PCR products; lane 7: standard fragment of <i>dspB</i> with <i>rsaA</i>.)
- 15:55, 25 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels (top)
- 18:39, 24 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 18:36, 24 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 18:33, 24 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 18:29, 24 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 18:02, 24 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 17:31, 24 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 17:27, 24 July 2011 (diff | hist) N File:Glogo1000.jpg (top)
- 17:26, 24 July 2011 (diff | hist) N File:Glogo975.jpg (top)
- 17:26, 24 July 2011 (diff | hist) N File:Glogo965.jpg (top)
- 19:03, 22 July 2011 (diff | hist) Team:Grinnell/Template/Test (Undo revision 51853 by Aaring (talk))
- 18:12, 22 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 17:57, 22 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 17:56, 22 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 16:39, 22 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 16:36, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 16:33, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 16:32, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 16:27, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels (Replaced content with "{{:Team:Grinnell/Template/Home}} <html> <body> <h1>Gel Pics</h1> <p>We collected more gel data than we could fit on one page. Please go on to one of the following pages:</p> ...")
- 16:20, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/Promoters (→2011.06.26-2011.07.02)
- 16:15, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/Promoters (→2011.06.19-2011.06.25)
- 16:11, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/Promoters (→Dates)
- 16:07, 22 July 2011 (diff | hist) N Team:Grinnell/Notebook/Gels/Promoters (Created page with "{{:Team:Grinnell/Template/Home}} {{:Team:Grinnell/Template/Gallery}} =Gels for Promoters= ==Dates==")
- 15:55, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/Esp (→2011.07.17-2011.07.23)
- 15:54, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/Esp (→2011.07.10-2011.07.16)
- 15:49, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/Esp (→2011.07.03-2011.07.09)
- 15:40, 22 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/Esp
- 19:46, 21 July 2011 (diff | hist) N Team:Grinnell/Notebook/Gels/Esp (Created page with "{{:Team:Grinnell/Template/Home}} {{:Team:Grinnell/Template/Gallery}} =Gels for Esp= ==2011.06.05-2011.06.11== <html> <table class="gallery" frame='void' rules='none'> <tr> ...")
- 19:30, 21 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/DspB (→2011.07.17-2011.07.23)
- 17:03, 21 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/DspB (→Gels for DspB)
- 16:51, 21 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels/DspB
- 16:46, 21 July 2011 (diff | hist) N Team:Grinnell/Notebook/Gels/DspB (Created page with "{{:Team:Grinnell/Template/Home}} {{:Team:Grinnell/Template/Gallery}} =Gels for DspB= <!--==Week X==")
- 16:31, 21 July 2011 (diff | hist) Team:Grinnell/Template/Gallery (top)
- 16:29, 21 July 2011 (diff | hist) N Team:Grinnell/Template/Gallery (Created page with "<html><head> <style type="text/css"> table.gallery{ border-collapse:collapse; background:#FFFFFF; } table.gallery tr td{ width:200px; text-align:center; padding-l...")
- 15:54, 21 July 2011 (diff | hist) Team:Grinnell
- 22:07, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 22:02, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 22:02, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
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- 21:55, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 21:03, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 20:52, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 20:52, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 20:51, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 20:51, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 20:49, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 19:35, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 19:34, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 19:32, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 19:29, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 19:29, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 19:19, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 17:12, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 17:04, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 16:55, 20 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 16:51, 20 July 2011 (diff | hist) Team:Grinnell/Attributions (→DspB References)
- 16:51, 20 July 2011 (diff | hist) Team:Grinnell/Attributions (→DspB References)
- 16:46, 20 July 2011 (diff | hist) Team:Grinnell/Attributions (→RsaA References)
- 16:43, 20 July 2011 (diff | hist) Team:Grinnell/Attributions (→DspB References)
- 15:57, 20 July 2011 (diff | hist) Team:Grinnell/Attributions
- 21:56, 19 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 21:50, 19 July 2011 (diff | hist) N File:20110718 optesprsaAGXIDTSMART optesprsaAC3.jpg (Lane 1: ladder; lanes 2-7: transformants that should carry optimized <i>esp</i> with <i>rsaA</i> from gel extraction in IDTSMART-AMP. Lanes 3, 4, and 6 have the desired gene sequence.) (top)
- 21:47, 19 July 2011 (diff | hist) N File:20110718 optespC3 optespPxylC3.jpg (Lane 1: ladder; lanes 2-4: transformants that should carry optimized <i>esp</i> in pSB1C3; lanes 5-10: transformants that should carry optimized <i>esp</i> behind P<sub>xyl</sub>. Lanes 5 and 7 have the desired gene sequence.) (top)
- 21:44, 19 July 2011 (diff | hist) N File:20110718 optespC3 optespPrsaAC3.jpg (Lane 1: ladder; lanes 2-4: transformants that should carry optimized <i>esp</i> in pSB1C3; lanes 5-10: transformants that should carry optimized <i>esp</i> behind P<sub>rsaA</sub>. Lane 9 has the desired gene sequence.) (top)
- 21:41, 19 July 2011 (diff | hist) N File:20110718 BBa OR PrsaA+optdspB+rsaA pMR10.jpg (Lane 1: ladder; lanes 2-5: transformants that should carry BBa_K081005, optimized <I>dspB</i>, and <i>rsaA</i> in pMR10; lanes 6-9: transformants that should carry P<sub>rsaA</sub>, optimized <I>dspB</i>, and <i>rsaA</i> in pMR10; lane 10: standard DNA fr)
- 21:09, 19 July 2011 (diff | hist) N File:20110715 Pxyl+optdspB+rsaA c9-14.jpg (Lane 1: ladder; lanes 2-7: transformants that should carry P<sub>xyl</sub>, optimized <i>dspB</i>, and <i>rsaA</i>; lane 8: standard fragment of optimized <i>dspB</i> with <i>rsaA</i>. None of the transformants carried the desired gene sequence.)
- 21:01, 19 July 2011 (diff | hist) N File:20110715 BBa+optdspB+rsaA.jpg (Lane 1: ladder; lanes 2-5: transformants that should carry BBa_K081005, optimized <i>dspB</i>, and <i>rsaA</i>; lane 6: standard optimized <i>dspB</i> with <i>rsaA</i> fragment. Lanes 3 and 5 carry the desired combination of genes.)
- 20:57, 19 July 2011 (diff | hist) N File:20110714 WTdspB+Pxyl.jpg (Lane 1: standard optimized <i>dspB</i> with <i>rsaA</i> from PCR; lanes 2,3: transformants that should carry WT <i>dspB</i> behind P<sub>xyl</sub>; lane 4: ladder. No successful transformations.)
- 20:54, 19 July 2011 (diff | hist) N File:20110714 optespIDT.jpg (Lane 1: ladder; lanes 2-9: transformants that should carry optimized <i>esp</i> in IDTSMART-AMP; lane 10: confirmation of digest of miniprep of strain carrying BBa_K081005, WT <i>esp</i>, and <i>rsaA</i> in pMR10. Lanes 4, 7, and 9 show successful transfo) (top)
- 20:51, 19 July 2011 (diff | hist) N File:20110714 optdspB+rsaA+Pxyl c5-8.jpg (Lane 1: ladder; lanes 2-5: transformants that should carry P<sub>xyl</sub>, optimized <I>dspB</i>, and <I>rsaA</i>; lane 6: standard optimized <i>dspB</i> with <i>rsaA</i> from PCR. None of these colonies were successful.)
- 20:41, 19 July 2011 (diff | hist) N File:20110714 optdspB+rsaA+PrsaA OR Pxyl.jpg (Lane 1: ladder; lanes 2-5: transformants that should carry P<sub>rsaA</sub>, optimized <i>dspB</i>, and <i>rsaA</i>; lanes 6-9: transformants that should carry P<sub>xyl</sub>, optimized <i>dspB</i>, and <i>rsaA</i>; lane 10: standard optimized <I>dspB</I)
- 20:36, 19 July 2011 (diff | hist) N File:20110713 optdspBIDT optespIDT.jpg (<html> Lanes 1-4: transformants that should carry optimized <i>dspB</i> in IDTSMART-AMP; lanes 5,8-10: transformants that should carry optimized <i>esp</i> in IDTSMART-AMP; lane 6: ladder; lane 7: optimized esp standard. None of these transformants ended ) (top)
- 20:30, 19 July 2011 (diff | hist) File:20110713 BD1+pMR10 DRDX.jpg
- 20:29, 19 July 2011 (diff | hist) N File:20110713 optespC3 optespIDTdF.jpg (<html> Lane 1: ladder; lanes 2-5: colony PCR of transformants that should carry the optimized <i>esp</i> in pSB1C3; lane 6: follow up digest of miniprep of transformant that may carry optimized <i>esp</i> in IDTSMART-AMP. None of these colonies were succe) (top)
- 20:20, 19 July 2011 (diff | hist) N File:20110713 BD1+pMR10 DRDX.jpg (<html> Gel of colony PCR of transformants that should carry BBa_K081005, WT esp, and rsaA in pMR10, P<sub>xyl</sub>, WT esp, and rsaA in pSB1C3, or P<sub>rsaA</sub>, WT esp, and rsaA in pSB1C3. Lane 1: ladder; lanes 2-5: BBa_K081005, WT esp, and rsaA in p)
- 18:52, 19 July 2011 (diff | hist) N File:20110712 optdspB+rsaAc1-4 optdspB.jpg (<html> Gel of colony PCR of transformants carrying optimized <i>dspB</i> and <i>rsaA</i> C-terminal. Lane 1: ladder; lanes 2-5: transformation colonies 1-4; lane 6: <i>dspB</i> standard from PCR of WT <i>dspB</i>. </html>)
- 18:50, 19 July 2011 (diff | hist) File:20110712 dspB+Pxyl forGX.jpg
- 18:50, 19 July 2011 (diff | hist) File:20110711 c9 10 12 15 from20110707transf-Pxyl+dspBWT.jpg
- 18:48, 19 July 2011 (diff | hist) N File:20110712 dspB+Pxyl forGX.jpg (<html> Gel for extraction of WT dspB behind P<sub>xyl</sub>. Lane 1: ladder; lanes 2,3: miniprep digest. </html>)
- 18:45, 19 July 2011 (diff | hist) N File:20110711 c9 10 12 15 from20110707transf-Pxyl+dspBWT.jpg (<html> Gel of colony PCR of transformants that should carry WT dspB behind the P<sub>xyl</sub> promoter. Lanes 1-4: colonies 9, 10, 12, and 15 from transformation plate; lane 5: dspB WT standard from PCR. </html>)
- 18:42, 19 July 2011 (diff | hist) N File:20110616 promoterPostCleanupCrop.jpg (top)
- 18:40, 19 July 2011 (diff | hist) File:20110616 promoterPostCleanup.jpg (uploaded a new version of "File:20110616 promoterPostCleanup.jpg": Properly cropped this time) (top)
- 17:10, 19 July 2011 (diff | hist) Team:Grinnell/Attributions (→RsaA References)
- 17:00, 19 July 2011 (diff | hist) Team:Grinnell/Attributions (→RsaA References)
- 16:46, 19 July 2011 (diff | hist) Team:Grinnell/Attributions
- 16:42, 19 July 2011 (diff | hist) Team:Grinnell/Project/RsaA
- 15:47, 19 July 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 15:15, 19 July 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 15:15, 19 July 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 20:07, 18 July 2011 (diff | hist) N Team:Grinnell/Project/RsaA (Created page with "{{:Team:Grinnell/Template/Home}} =RsaA= <!--There isn't any text here b/c I haven't had time to fill it in yet-->")
- 20:01, 18 July 2011 (diff | hist) Team:Grinnell
- 20:00, 18 July 2011 (diff | hist) Team:Grinnell
- 20:52, 15 July 2011 (diff | hist) Team:Grinnell/Recipes
- 20:51, 15 July 2011 (diff | hist) Team:Grinnell/Recipes
- 20:46, 15 July 2011 (diff | hist) Team:Grinnell/Safety
- 16:27, 15 July 2011 (diff | hist) Team:Grinnell
- 16:25, 15 July 2011 (diff | hist) Team:Grinnell
- 16:25, 15 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:11, 12 July 2011 (diff | hist) Team:Grinnell/Notebook (→Week 6)
- 21:10, 12 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 16:43, 12 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 16:26, 12 July 2011 (diff | hist) N File:20110710 optGenes+C3.jpg (<html> Gel of transformants with synthesized <i>dspB</i> and <i>esp</i> with codons optimized for expression in <i>Caulobacter</i> after ligation into pSB1C3. Lane 1: ladder; lanes 2-5: optimized <i>dspB</i>; lanes 6-9: optimized <i>esp</i>. Lane 4 shows )
- 16:21, 12 July 2011 (diff | hist) N File:20110709dspB+pxyl Colonies5-8.jpg (<html> Gel of more transformants of <i>dspB</i> with P<sub>xyl</sub>. Lane 1: ladder; lanes 2-5: transformants; lane 6: standard <i>dspB</i> with no promoter. </html>)
- 16:18, 12 July 2011 (diff | hist) N File:20110709 MorePromoter combo 2.jpg (<html> Gel of transformants that should carry <i>esp</i> + <i>rsaA</i> combo insert behind P<sub>rsaA</sub> run against standard combo. Lane 1: ladder; lanes 2-4: combo from miniprep digest and gel extract insert; lanes 5-7: combo from PCR product insert;) (top)
- 16:14, 12 July 2011 (diff | hist) N File:20110709 MorePromoter combo 1.jpg (<html> Gel of more transformants that should have <i>esp</i> + <i>rsaA</i> combo inserted behind P<sub>xyl</sub>. Lane 1: ladder; lanes 2-4: combo from miniprep digest and gel extract insert; lanes 5-7: combo from PCR insert; lane 8: confirmation of inser) (top)
- 16:02, 12 July 2011 (diff | hist) N File:20110708 Pxyl PrsaA combo.jpg (<html> Gel of transformants that should carry <i>esp</i> + <i>rsaA</i> behind a promoter. Lane 1: ladder; lanes 2-5: P<sub>rsaA</sub> with insert; lanes 6-9: P<sub>xyl</sub> with insert. None of these transformants show the desired insert. </html>) (top)
- 15:55, 12 July 2011 (diff | hist) N File:20110708 dspB+PrsaA Pxyl.jpg (<html> Gel of transformants that should contain both <i>dspB</i> and a promoter insert. Lane 1: ladder; lanes 2-4: <i>dspB</i> with P<sub>rsaA</sub>; lanes 5-7: <i>dspB</i> with P<sub>xyl</sub>; lane 8: standard <i>dspB</i> with no promoter. </html>)
- 15:51, 12 July 2011 (diff | hist) N File:20110708 dspB+promotersVSdspB std.jpg (<html> Gel showing <i>dspB</i> with various promoter inserts as contrasted to a standard <i>dspB</i> with no promoter. Lane 1: dspB (WT) + P<sub>rsaA</sub>; lane 2: with P<sub>xyl</sub>; lanes 3,4: with BBa_K081005; lane 5: standard <i>dspB</i>; lane 6: l)
- 15:44, 12 July 2011 (diff | hist) N File:20110708 BBaGel.jpg (<html> Gel of transformation products of <i>esp</i> + <i>rsaA</i> insert into plasmid containing promoter BBa_K081005. Lane 1: ladder; lanes 2-5: transformants using gel extracted combo gene; lanes 6-9: transformants using PCR product for combo insert. </) (top)
- 15:38, 12 July 2011 (diff | hist) N File:20110707 UBC dspB DigestXP.jpg (Lane 1: ladder; lane 2: digest with XbaI and PstI of WT dspB. Gel confirms that the digest was effective) (top)
- 16:15, 8 July 2011 (diff | hist) N File:20110707 UBC dspB transformation results colonies 1 2 3.jpg (<html> Gel proving the successful transformation of <i>dspB</i> containing plasmid into <i>E. coli</i> Top10. Lane 1: ladder; lanes 2-4: digests (EcoRI and PstI) of miniprep DNA from overnights of transformants. There is a clear band at the appropriate si)
- 16:12, 8 July 2011 (diff | hist) N File:20110707 GXandPCR combo.jpg (<html> Gel for gel extraction of <i>esp</i> and <i>rsaA</i> combination and checking the identity of PCR product. Lane 1: ladder; lanes 3,4: digest of miniprep DNA for gel extraction; lane 6: PCR product. Gel is somewhat messy, but appropriately sized ban) (top)
- 21:14, 7 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 21:13, 7 July 2011 (diff | hist) Team:Grinnell/Template/Home
- 20:58, 7 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 20:57, 7 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 20:55, 7 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 20:53, 7 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 20:37, 7 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 20:37, 7 July 2011 (diff | hist) Team:Grinnell/Template/Test
- 19:00, 7 July 2011 (diff | hist) Team:Grinnell/Safety
- 16:50, 7 July 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 16:39, 7 July 2011 (diff | hist) Team:Grinnell/Notebook (→Week 6)
- 16:30, 7 July 2011 (diff | hist) Team:Grinnell/Notebook (→Week 6)
- 16:23, 7 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 16:05, 7 July 2011 (diff | hist) Team:Grinnell/Notebook (→Week 6)
- 16:04, 7 July 2011 (diff | hist) Team:Grinnell/Notebook (→Week 6)
- 16:03, 7 July 2011 (diff | hist) Team:Grinnell/Applications (→Applications) (top)
- 15:28, 7 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 15:22, 7 July 2011 (diff | hist) N File:20110706 MoreColoniesFollowup.jpg (<html> Follow up gel of lanes 5 and 6 from the previous gel, redigested for a longer period of time. Lane 1: ladder; lane 2: digest of miniprep product from colony that should contain an insert of <i>rsaA</i> into plasmid already containing <i>esp</i> (pr) (top)
- 15:17, 7 July 2011 (diff | hist) N File:20110706 MoreColonies.jpg (<html> Gel of restriction digests (EcoRI and PstI) of miniprepped DNA from more transformants from the weekend's transformations. Lane 1: ladder; lanes 2-5: colonies that should contain <i>rsaA</i> inserted into pSB1C3 containing <i>esp</i>; lanes 6-9: co) (top)
- 15:09, 7 July 2011 (diff | hist) N File:20110706 rsaAGelExtractPrep.jpg (<html> Gel of digests of pSB1C3 containing <i>rsaA</i> C-terminal in preparation for gel extraction. Lane 1: ladder; lanes 3,4: digest with XbaI and PstI; lanes 6,7: digest with EcoRI and XbaI. </html>) (top)
- 14:10, 7 July 2011 (diff | hist) N File:20110706 espGelExtractPrep.jpg (<html> Gel of digested pSB1C3 containing <i>esp</i> for gel extraction. Lane 1: ladder; lanes 2,3: digest with EcoRI and SpeI; lanes 5,6: digest with SpeI and PstI. </html>) (top)
- 22:37, 6 July 2011 (diff | hist) Team:Grinnell/Project/DspB (→DspB)
- 22:29, 6 July 2011 (diff | hist) Team:Grinnell/Project/DspB
- 22:29, 6 July 2011 (diff | hist) Team:Grinnell/Project/DspB
- 22:27, 6 July 2011 (diff | hist) Team:Grinnell/Team
- 22:59, 5 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 22:43, 5 July 2011 (diff | hist) N File:20110705 Tests.jpg (<html> Lane 1: ladder; lane 2: digest of miniprep of transformation product of insert of <i>esp</i> from gel extraction into plasmid containing <i>rsaA</i>; lanes 3,4: ligations gel extracted <i>esp</i> and <i>rsaA</i> and plasmid containing the other gen) (top)
- 22:33, 5 July 2011 (diff | hist) N File:20110705 TestGelExtract.jpg (<html>Lane 1: ladder; lane 2: ligation of <i>esp</i> and <i>rsaA</i> C-terminal fragments from gel extraction. No <i>rsaA</i> appears to be present, nor any ligation, only <i>esp</i>.</html>) (top)
- 20:52, 5 July 2011 (diff | hist) N File:20110705 notCombo.jpg (<html>Digested miniprep samples of overnight cultures of transformation product that may contain <i>esp</i> and <i>rsaA</i>. Lane 1: <i>esp</i> standard; lanes 2,3: <i>rsaA</i> from gel extraction inserted into pSB1C3 containing <i>esp</i> from plates spr) (top)
- 16:41, 5 July 2011 (diff | hist) Team:Grinnell/Parts
- 20:29, 4 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 20:20, 4 July 2011 (diff | hist) N File:20110704 combo.jpg (PCR test of transformation products of insertion of gel extracted DNA into plasmid containing another gene. Lane 1: ladder; lanes 2-4: insertion of <html><i>rsaA</i> C-terminal into plasmid containing <i>esp</i>; lanes 5-7: insertion of <i>esp</i> into pl) (top)
- 23:02, 1 July 2011 (diff | hist) N Team:Grinnell/Sandbox/Gel (Created page with "{{:Team:Grinnell/Template/Home}} <html><head> <style type="text/css"> </style> </head> <body> <h1>Gel Pictures</h1> <p>This exists to play with now</p> </body></html>") (top)
- 22:59, 1 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 22:59, 1 July 2011 (diff | hist) N File:20110701 combo2.jpg (Gel 2 of 2. PCR of transformation products of ''rsaA'' C-terminal insert into pSB1C3 containing ''esp''. Lane 1: ladder; lanes 2-4: ligation 2, colonies 4-6; lanes 5-10: ligation 3, colonies 1-6. All the bands here are correct for ''esp'', but not the co) (top)
- 22:55, 1 July 2011 (diff | hist) N File:20110701 combo1.jpg (Gel 1 of 2. PCR of transformation products of ''rsaA'' C-terminal insert into pSB1C3 containing ''esp''. Lane 1: ladder; lanes 2-7: ligation 1, colonies 1-6; lanes 8-10: ligation 2, colonies 1-3. The complete lack of DNA fragments here means that these l) (top)
- 16:31, 1 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 16:26, 1 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 16:25, 1 July 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 21:39, 30 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 21:38, 30 June 2011 (diff | hist) N File:20110630 espORcombo.jpg (Verification of presence of lack of ''rsaA'' C-terminal insert in pSB1C3 containing ''esp''. Lane 1: ladder; lane 2: digested PCR of possible insert; lane 3: standard digested ''esp''. The band is faint, but there appears to be a band at the same height ) (top)
- 17:43, 30 June 2011 (diff | hist) File:20110629 PromoterInserts1.jpg (top)
- 17:43, 30 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 17:43, 30 June 2011 (diff | hist) N File:20110629 PromoterInserts Both.jpg (Gel 3 of 3. PCR product of transformation colonies that should contain an insert of either P<html><sub>xyl</sub> or P<sub>rsaA</sub></html> into pSB1C3 containing ''esp'' and ''rsaA'' C-terminal. Lane 1: ladder; lanes 2,3: P<html><sub>xyl</sub> insert P) (top)
- 17:31, 30 June 2011 (diff | hist) N File:20110629 PromoterInserts PrsaA.jpg (Gel 2 of 3. PCR of transformation cells that should contain an insert of P<html><sub>rsaA</sub></html> into pSB1C3 containing ''esp'' and ''rsaA'' C-terminal. Lane 1: ladder; lanes 2-5: PCR with plasmid primers VF2 and VR; lanes 6-9: PCR with promoter spe) (top)
- 17:18, 30 June 2011 (diff | hist) N File:20110629 PromoterInserts1.jpg (PCR of transformation cells that should contain an insert of P<html><sub>xyl</sub></html> into pSB1C3 containing ''esp'' and ''rsaA'' C-terminal. Lane 1: ladder; lanes 2-5: PCR with plasmid primers VF2 and VR; lanes 6-9: PCR with promoter specific forward)
- 16:51, 30 June 2011 (diff | hist) N File:20110629 BBa K081005.jpg (Lane 1: ladder; lane 2: colony PCR product for promoter BBa_K081005 in preparation for digestion and insertion into plasmid containing ''esp'' and ''rsaA'' C-terminal. Result is positive.) (top)
- 14:30, 29 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 14:29, 29 June 2011 (diff | hist) N File:20110628 TestDigest.jpg (Test to ensure all of our restriction enzymes are still active. Lane 1: ladder; lane 2: cut with Eco RI; lane 3: cut with XbaI; lane 4: standard uncut DNA fragment for lanes 2 and 3; lane 5: cut with SpeI; lane 6: cut with PstI; lane 7: standard uncut DN) (top)
- 19:50, 28 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 19:27, 28 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 19:13, 28 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 19:07, 28 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 18:43, 28 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 18:41, 28 June 2011 (diff | hist) Team:Grinnell/Recipes
- 18:37, 28 June 2011 (diff | hist) Team:Grinnell/Gallery (top)
- 18:37, 28 June 2011 (diff | hist) Team:Grinnell/Gallery
- 18:36, 28 June 2011 (diff | hist) Team:Grinnell/Gallery
- 18:36, 28 June 2011 (diff | hist) Team:Grinnell/Applications
- 18:35, 28 June 2011 (diff | hist) Team:Grinnell/Parts (→Parts)
- 18:34, 28 June 2011 (diff | hist) Team:Grinnell/Project
- 17:10, 28 June 2011 (diff | hist) Team:Grinnell/Gallery
- 16:02, 28 June 2011 (diff | hist) Team:Grinnell/Team
- 16:01, 28 June 2011 (diff | hist) Team:Grinnell/Team (→What we did)
- 15:58, 28 June 2011 (diff | hist) Team:Grinnell/Team (→What we did)
- 15:58, 28 June 2011 (diff | hist) Team:Grinnell/Team (→What we did)
- 15:57, 28 June 2011 (diff | hist) Team:Grinnell
- 15:41, 28 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 15:40, 28 June 2011 (diff | hist) N File:20110627 esprsaA+promoters 2.jpg (Gel 2 of 2. PCR products of transformation cells that should contain ''esp'' + ''rsaA'' C-terminal insert into plasmids containing various promoters. Lane 1: ladder; lanes 2-7: inserts into plasmid containing P<html><sub>rsaA</sub>; lane 8: standard P<sub) (top)
- 15:32, 28 June 2011 (diff | hist) N File:20110627 esprsaA+promoters 1.jpg (Gel 1 of 2. PCR products of transformation cells that should contain ''esp'' + ''rsaA'' C-terminal insert into plasmids containing various promoters. Lane 1: ladder; lanes 2-5: inserts into plasmid containing BBa_K081005; lane 6: standard BBa_K081005 con) (top)
- 02:18, 28 June 2011 (diff | hist) Team:Grinnell/Recipes
- 02:17, 28 June 2011 (diff | hist) Team:Grinnell/pxyl (top)
- 02:16, 28 June 2011 (diff | hist) Team:Grinnell/prsaA (top)
- 22:15, 27 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 22:14, 27 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 21:51, 27 June 2011 (diff | hist) Team:Grinnell/Notebook
- 21:49, 27 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 4)
- 21:48, 27 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 3)
- 01:07, 27 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 01:03, 27 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels (→Gel Pictures)
- 01:01, 27 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 21:18, 26 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 21:15, 26 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 21:14, 26 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 21:03, 26 June 2011 (diff | hist) File:20110617 Pro-esp-stop-rsaA.jpg (top)
- 20:31, 26 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 20:28, 26 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 20:25, 26 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 20:24, 26 June 2011 (diff | hist) Team:Grinnell/Notebook/Gels
- 20:23, 26 June 2011 (diff | hist) N Team:Grinnell/Notebook/Gels (Created page with "<html><head> <style type="text/css"> ul.cap li{ width-max:400px; } ul.cap{ float:left; } </style> </head> <body> <h2>Gel Pictures</h2> <h3>June 6 to 10</h3> <ul class="cap"> <li...")
- 23:00, 24 June 2011 (diff | hist) N File:20110623 esp+rsaA 246.jpg (Gel 2 of 2. Colony PCR of transformation products of insertions of ''esp'' from PCR into pSB1C3 containing ''rsaA'' C-terminal, insertions of ''rsaA'' C-terminal into pSB1C3 containing ''esp'', and ''esp'' and ''rsaA'' C-terminal digests from pSB1C3 in) (top)
- 23:00, 24 June 2011 (diff | hist) N File:20110623 esp+rsaA 135.jpg (Gel 1 of 2. Colony PCR of transformation products of insertions of ''esp'' from PCR into pSB1C3 containing ''rsaA'' C-terminal, insertions of ''rsaA'' C-terminal into pSB1C3 containing ''esp'', and ''esp'' and ''rsaA'' C-terminal digests from pSB1C3 in) (top)
- 22:52, 24 June 2011 (diff | hist) N File:20110624 BBa K081005 esp rsaA.jpg (Colony PCR amplified DNA fragments of transformation products of inserts into plasmid containing existing Biobrick promoter BBa_K081005. Lane 1: ladder; lanes 2-7: PCR products; lane 8: standard DNA fragment identified as containing BBa_K081005. The lack ) (top)
- 22:48, 24 June 2011 (diff | hist) N File:20110624 Pxyl esp rsaA.jpg (Colony PCR products of transformation products of insertion of ''esp'' + ''rsaA'' into pSB1C3 containing ''Caulobacter'' inducible promoter Pxyl (induced in presence of xylene). Lane 1: ladder; lanes 2-7: PCR products; lane 8: standard PCR product of pSB) (top)
- 22:45, 24 June 2011 (diff | hist) N File:20110624 PrsaA esp rsaA.jpg (Colony PCR products of transformation products of insertions of ''esp'' + ''rsaA'' C-terminal into plasmid containing ''Caulobacter'' constitutive promoter PrsaA. Lane 1: ladder; lanes 2-7: PCR products; lane 8: standard PCR product of pSB1C3 containing o) (top)
- 22:41, 24 June 2011 (diff | hist) N File:2011062 BBa K081005 esp rsaA.jpg (Colony PCR amplified DNA fragments of inserts into plasmid containing existing Biobrick promoter BBa_K081005. Lane 1: ladder; lanes 2-7: PCR products; lane 8: standard DNA fragment identified as containing BBa_K081005. The lack of DNA may be due to a br) (top)
- 23:44, 23 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 4)
- 22:55, 23 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 4)
- 22:15, 23 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 4)
- 22:05, 23 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 4)
- 21:41, 22 June 2011 (diff | hist) N File:20110622 PromotersGel2.jpg (Lane 1: ladder; lanes 2-4: plasmid PCR product of various promoter insertions into pSB1C3. Smearing due to leftover circular plasmid. Insertions seem to have been successful.) (top)
- 21:39, 22 June 2011 (diff | hist) N File:20110621 rsaA.jpg (Lanes 1-3 and 5-7: plasmid PCR of colonies that should be carrying ''rsaA'' C-terminal insert in pSB1C3; lane 4: ladder. Lanes 3 and 4 seem to show insert at significant concentrations.) (top)
- 21:11, 22 June 2011 (diff | hist) N File:20110621 Promoter-esp-Stop-rsaA-pMR10.jpg (Lanes 1-3 and 5-8: plasmid PCR of transformed cells that should carry the Promoter-esp-stop-rsaA into plasmid pMR10; lane 4: ladder. Only lane 3 shows any success.) (top)
- 21:02, 22 June 2011 (diff | hist) N File:20110621 espAndCombo.jpg (Lanes 1-6: plasmid PCR of transformed cells that should carry the desired ''esp'' insert; lane 7: ladder; lanes 8-10: plasmid PCR of transformed cells that should be carrying ''esp'' + ''rsaA'' C-terminal insert. A few of the ''esp'' inserts succeeded, b) (top)
- 20:56, 22 June 2011 (diff | hist) N File:20110621 BBaGel1.jpg (Lane 1: ladder; lane 2: digest of miniprep from overnights that should carry the desired promoter insert. Digest shows one band: the linearized plasmid.) (top)
- 20:54, 22 June 2011 (diff | hist) N File:20110620 promotorsTransformationGel.jpg (Presence of two small bands shows contamination of samples. Lane 1: ladder; lanes 2-5: plasmid PCR of transformation survivors.) (top)
- 19:47, 22 June 2011 (diff | hist) Team:Grinnell/Notebook
- 22:42, 21 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 3)
- 22:41, 21 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 22:39, 21 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 22:31, 21 June 2011 (diff | hist) Team:Grinnell/Recipes
- 21:03, 21 June 2011 (diff | hist) Team:Grinnell/Recipes
- 21:42, 17 June 2011 (diff | hist) Team:Grinnell/Notebook
- 21:36, 17 June 2011 (diff | hist) N File:20110617 rsaA&esp.jpg (Lane 1: ladder; lanes 2,3: PCR product of ''rsaA'' using new primers from chromosomal ''Caulobacter'' DNA; lane 4: PCR product of ''esp'' using new primers from chromosomal ''S. epidermidis'' DNA.) (top)
- 21:32, 17 June 2011 (diff | hist) N File:20110617 Pro-esp-stop-rsaA.jpg (Lane 1: ladder; lanes 2-4 and 6-8: PCR product of transformation survivors; lane 5: control DNA of esp + rsaA.)
- 21:30, 17 June 2011 (diff | hist) N File:20110616 promoterPostCleanup.jpg
- 21:18, 17 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 3)
- 19:12, 17 June 2011 (diff | hist) Team:Grinnell/Notebook
- 17:22, 17 June 2011 (diff | hist) Team:Grinnell/Notebook
- 16:49, 17 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 3)
- 19:53, 16 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 19:51, 16 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 19:47, 16 June 2011 (diff | hist) Team:Grinnell/Attributions/Sponsors
- 19:43, 16 June 2011 (diff | hist) Team:Grinnell/Notebook
- 19:36, 16 June 2011 (diff | hist) File:20110614 PCR135.jpg (top)
- 19:26, 16 June 2011 (diff | hist) Team:Grinnell/Notebook
- 19:17, 16 June 2011 (diff | hist) File:20110616 promotorGel.jpg (top)
- 19:17, 16 June 2011 (diff | hist) Team:Grinnell/Notebook
- 19:16, 16 June 2011 (diff | hist) Team:Grinnell/Notebook
- 18:58, 16 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 18:54, 16 June 2011 (diff | hist) N File:20110616 promotorGel.jpg (Lane 1: ladder; lane 2: PrsaA PCR product; lane 3: Pxyl PCR product)
- 17:17, 16 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 17:15, 16 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 17:09, 16 June 2011 (diff | hist) Team:Grinnell/Attributions
- 17:08, 16 June 2011 (diff | hist) Team:Grinnell/Team
- 17:06, 16 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 17:06, 16 June 2011 (diff | hist) Team:Grinnell/Project
- 17:05, 16 June 2011 (diff | hist) Team:Grinnell/Safety
- 17:05, 16 June 2011 (diff | hist) Team:Grinnell/Parts
- 17:04, 16 June 2011 (diff | hist) Team:Grinnell/Parts
- 17:04, 16 June 2011 (diff | hist) Team:Grinnell/Project
- 17:04, 16 June 2011 (diff | hist) Team:Grinnell/Safety
- 17:01, 16 June 2011 (diff | hist) Team:Grinnell/Notebook
- 17:01, 16 June 2011 (diff | hist) Team:Grinnell/Notebook
- 16:53, 16 June 2011 (diff | hist) N Team:Grinnell/Attributions/Sponsors (Created page with "{{:Team:Grinnell/Template/Home}}")
- 16:52, 16 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 16:50, 16 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 16:49, 16 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 16:47, 16 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 16:50, 15 June 2011 (diff | hist) N Team:Grinnell/Sandbox (Created page with "{{:Team:Grinnell/Template/Test}} Week 1 PCR primers designed according to Biobrick specifications and ordered for rsaA C-terminal and WT esp. Because of the discrepancy in GC c...") (top)
- 16:48, 15 June 2011 (diff | hist) N Team:Grinnell/Template/Test (Created page with "<html><head> <title>Grinnell Menubar</title> <style type="text/css"> /*Roughly inspired by JHU iGEM 2010 and Team Heidelberg 2008, who copied their menu from Cesar Daniel (...")
- 02:49, 15 June 2011 (diff | hist) N File:20110614 Promoters.jpg (Gel of promoters taken from ''Caulobacter'': Pxyl (inducible) and PrsaA (constitutive).) (top)
- 02:48, 15 June 2011 (diff | hist) N File:20110614 PCR246.jpg (Gel 2 of 2 for June 14 for Alex and Qimeng.) (top)
- 02:45, 15 June 2011 (diff | hist) N File:20110614 PCR135.jpg (Gel 1 of 2 for June 14 for Alex and Qimeng.)
- 21:18, 12 June 2011 (diff | hist) Team:Grinnell/Gallery
- 19:36, 12 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 18:51, 12 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 18:46, 12 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 18:41, 12 June 2011 (diff | hist) Team:Grinnell/Notebook
- 00:25, 11 June 2011 (diff | hist) Team:Grinnell/Notebook
- 00:25, 11 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 00:24, 11 June 2011 (diff | hist) Team:Grinnell/Template/Home
- 22:42, 10 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 22:42, 10 June 2011 (diff | hist) Team:Grinnell/Notebook/Protocols
- 22:16, 10 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 2)
- 22:11, 10 June 2011 (diff | hist) Team:Grinnell/Notebook (→Week 2)
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