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TB buffer for competent cells (Inoue)

Summary

SOB Medium. Used in growing bacteria for preparing chemically competent cells

Ingredients

• yeast extract
• tryptone
• NaCl
• KCl
• MgSO₄

Per liter:

• 5 g yeast extract (0.5% w/v)
• 20 g tryptone (2% w/v)
• 0.584 g NaCl (10 mM final)
• 0.186 g KCl (2.5 mM final)
• 2.4 g MgSO₄ (20 mM final)

Note: Some formulations of SOB use 10 mM MgCl₂ and 10 mM MgSO₄ instead of 20 mM MgSO₄.

SOB medium is also available dry premixed from Difco, 0443-17.

Adjust to pH 7.5. This requires approximately 25 ml of 1M NaOH per liter.

Autoclave.

Source

Adapted From:

Open Wet Ware

Trivia

SOB supposedly stands for super optimal broth.