Team:Caltech/Week 9

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Caltech iGEM 2011

Project

Data

Parts

Team

Notebook

Biosafety

Human Impact

References

Support

August 8

Testing for biofilms grown on 96-well plate and Erlenmeyer flasks with crystal violet
PCR of 16s
Transformation of I732019, I732005, J23119 from source plate in XL-10 gold.
Re-assembly of DDT gene using alternate method

Results

There were biofilms on all surfaces.

96-well plate with XL-10 biofilm grown for two days, visualized with 1% crystal violet

Glass beads with XL-10 biofilm grown for two days, visualized with 1% crystal violet

Got successful amplification of 16s in LA River sample 9-9. In both gels, the controls had no bands and all other sample lanes had no bands.