Team:Tokyo Metropolitan/Notebook/S01

From 2011.igem.org

Revision as of 11:13, 5 October 2011 by Rosanium (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

-Speeding Up-

Made agar plates

Made each five agar plates contained kanamycin and chloramphenicol.

-Visualization-

Digestion,electrophoresis and gel extraction J23100,I714051.

Confirm plasmid extraction was succeeded

Concentration of DNA solution(J23100,I714051) will measure tomorrow.


@DNA refine(OriTr+GFP,OriT)

electrophoresis and gel extract restriction enzymed sample(8/31).


@ligation

considering a small quantity of refined DNA, change reagent composition.


I. ligate solution

 oriTr:1μl

 oriTr+GFP:12μl

 T4:1μl

 ×10 buffer:2μl


II. control solution

 DW:12μl

 oriTr:1μl

 T4:1μl

 ×10 buffer:2μl