Team:Kyoto

From 2011.igem.org

Revision as of 21:32, 27 September 2011 by V08ken (Talk | contribs)

京都ホーム.jpg

Contents

Summary

We create new E.coli which hunt and eat insects. it is named Carnivorous E.coli. Carnivorous E.coli emits light when hungry, and insects come near it attracted by the light. Then, it secretes viscous material to catch the insects. The insects caught are solved by protease and chitinase that Carnivorous E.coli secretes also. So, Carnivorous E.coli can hunt!

Hunger

It is a burden for the E.coli to emit light. This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough.


Luminescence

There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone. Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from 2010 Cambridge.

Digestion

In this part, we aimed to create E.coli which digest bugs and survive under the nitrogen-poor condition. Digestion is accomplished by protease and chitinase. We will induce these enzymes and assay them.

Achievement

Human Practice

Reference