"
Violacein
We plan to finish ligating the 5 violacein expression cassettes into the PRS416 Yeast Vector and transform it into a URA3 Knockout yeast strain.
We plan to carry out the random ligation to make the promoter/violacein ORF/3'UTR/loxPsym combinatorial library and transform into synIXR yeast to get varying levels of violacein expression.
