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Team:Groningen/project characterisation mu
From 2011.igem.org
Memory Units
cI Autoinducing Loops
The autoinducing loop driven by cI will function as our first memory unit. We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we meassured the fluorescence in uninduced cells with a flow cytometer.
