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Team:Paris Bettencourt/Experiments
From 2011.igem.org
Revision as of 16:07, 19 September 2011 by BaptisteCouly (Talk | contribs)
Experiments
You can find here all the experiments we did in the lab! The data is organized by design and you will be able to find both the characterization steps and our diffusion experiments.
Preliminaries experiments
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Preliminaries experiments: go back and see how we re-did the experiment of the original nanotubes paper. |
Our major designs
These constructs took most of our time this summer and we really put some effort in their construction. See how they turned out.
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YFP concentration: this design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins. |
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T7 RNA polymerase diffusion: in this design, we used the T7 polymerase both as the transfer molecule and as the auto-amplification system. |
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tRNA amber diffusion: the tRNA amber is the smallest molecule we are trying to get pass the nanotubes. |
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ComS diffusion: we took advantage of a switch already existing in B.Subtilis (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes. |
Other designs
In addition to our major constructs, we found along the summer several other ideas to take advantage of already existing systems to test the nanotube network. Here are the experiments we did regarding those designs.
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ComS diffusion: we took advantage of a switch already existing in B.Subtilis (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes. |
