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• Home
• Presentation
  • Overview
  • Potential applications
  • Collaborations
  • The team
  • Acknowledgements →
    • Host laboratory
    • Sponsors
• Designs
  • Designs overview & concepts
  • Designs list
• Modeling
  • General overview
  • Modeling results for our designs →
    • The basics
    • Our assumptions
    • T7 polymerase diffusion
    • tRNA diffusion
    • ComK/ComS system
    • Parameters
  • Results of our passive diffusion model
  • Toward assisted diffusion
• Data
• Results
  • Achievements
  • Methodologies →
    • Microscopy
    • Micro-fluidic
    • Integration in B.subtilis
    • Kinetics
  • Parts list
  • Notebook
• Human practice & Safety
  • Human practice
  • Safety
  • Brainstorming
  • Bonus track

Experiments

You can find here all the experiments we did in the lab! The data is organized by design and you will be able to find both the characterization steps and our diffusion experiments.

Preliminaries experiments

Preliminaries experiments: go back and see how we re-did the experiment of the original nanotubes paper.

Our major designs

	YFP concentration: this design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins.
	T7 RNA polymerase diffusion: in this design, we used the T7 polymerase both as the transfer molecule and as the auto-amplification system.
	tRNA amber diffusion: the tRNA amber is the smallest molecule we are trying to get pass the nanotubes.
	ComS diffusion: we took advantage of a switch already existing in B.Subtilis (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes.