Team:Imperial College London/Protocols Auxin

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Protocols

This page lists all the protocols used in our project. We have classified them into five main categories as follow.




Auxin Xpress

Effect of Auxin on Plants


Split Root Experiment


Salkowski


To make reagent:

1. Dissolve 0.811g of anhydrous FeCl3 in 10ml H2O to obtain 0.5M solution

2. Add 1ml of FeCl3 0.5M solution to 50ml of 35% HClO4

3. store at room temperature in absence of sunlight

To perform the assay:

1. Measure the OD of your auxin producing cells and control cells at 600 nm to account for any difference in growth.

2. Spin down each cell sample and take an aliquot of supernatant to filter with ...

3. Add salkowski reagent to the filtered supernatant in a ratio of 2:1

4. Leave in the dark for 25-30 mins and then measure OD at 530 nm. (You should be able to see a visible colour change to pink/red if auxin is present.

HPLC


Soil Erosion Experiment